GGDB:

この酵素は、供与体基質UDP-GalNAcからタンパク質のセリンまたはスレオニン残基にGalNAcを転移する活性を持つ。反応生成物は広くTn-抗原として知られているGalNAc-alpha-O-Ser/Thr構造で、ムチン型O-グリカンの根本の構造である。受容体基質であるタンパク質の典型はムチンである。このアイソザイムはこのファミリーの6番目のメンバーである。このアイソザイムはGALNT3と高度に相同な遺伝子として発見されたが、その機能はGALNT3と重複していない。

Keyword: O-グリカン , Tn抗原 , ムチン , 腫瘍マーカー

HO-Ser/Thr	UDP-GalNAc	GalNAc(alpha1-O)-Ser/Thr

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase

GGDB Symbol

GALNT6 Alias

Designation

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6

Organism

Homo sapiens

GeneID

11226

HGNC

GALNT6 (4128)

mRNA

BC035822

map

12q13

Protein

AAH35822

EC#

2.4.1.41

CAZy

GT27

GlyCosmos Glycogenes

GALNT6

OMIM

605148

Disease name

GDGDB

Human Protein Atlas

ENSG00000139629

Mus musculus

mRNA	NM_172451
Protein	NP_766039
MGI	47983

Caenorhabditis elegans

mRNA	NM_067320
Protein	NP_499721

Drosophila melanogaster

mRNA	NM_166188
Protein	NP_725602

J. Biol. Chem. 1999-09-01 ;274(36):25362-70.

Cloning and characterization of a close homologue of human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy.

Bennett EP. et al. PMID:10464263

DONOR : UDP-GalNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	HO-Ser/Thr	UDP-GalNAc	GalNAc(alpha1-O)-Ser/Thr

table II Kinetic constants of purified recombinant GalNAc-transferases
Peptide (sequence)	apparent Vmax (nmol/min/？g)
Fibronectin (Ac-PFVTHPGYD)	3.64
HIV gp120 (Ac-CIRIQRGPGRAFVTIGKIGNMR)	0.66
Prion-a (KQHTVTTTTKGEN)	0.40
Zonadhesin (PTERTTTPTKRTTTPTIR)	0.30

table 1 Expression of human recombinant GalNAc-transferases in Sf9 cells
Peptide (sequence)	specific activity (milliunits/ml)
Muc1a peptide (AHGVTSAPDTR)	4.69
Muc2 peptide (PTTTPISTTTMVTPTPTPTC)	3.56
Fibronectin peptide (Ac-PFVTHPGYD)	1.59
EA2 peptide (PTTDSTTPAPTTK)	1.59
HIVHIB gp120 peptide (Ac-CIRIQRGPGRAFVTIGKIGNMR)	0.88
Muc7 peptide (Ac-CPPTPSATTPAPPSSSAPPETTAA)	0.69
OSM fragment peptide (LSESTTQLPGGGPGCA)	0.50
Muc1b peptide (RPAPGSTAPPA)	0.15
hCG-b peptide (PRFQDSSSSKAPPPSLPSPSRLPG)	0.00

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

J. Biol. Chem. 1999-09-01 ;274(36):25362-70.

Cloning and characterization of a close homologue of human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy.

Bennett EP. et al. PMID:10464263

adrenal glandsbone marrowbraincolonheartintestinekidneyliverlungovarypancreasperipheral blood leukocyteplacentaprostateskeletal musclespinal cordspleenstomachtestisthymusthyroid glandtrachea

Provided by GOA

Function
carbohydrate binding
metal ion binding
polypeptide N-acetylgalactosaminyltransferase activity

Process
O-glycan processing
protein O-linked glycosylation

Component
Golgi apparatus
Golgi membrane
integral component of membrane
perinuclear region of cytoplasm

GALNT6

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase

Orthologous Gene

Mus musculus

Caenorhabditis elegans

Drosophila melanogaster

Acceptor Substrates (Reference)

Cloning and characterization of a close homologue of human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy.

DONOR : UDP-GalNAc

Substrate Transferred

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

Cloning and characterization of a close homologue of human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy.

Gene Ontology