GGDB:

β3GnT5 (B3GNT5) is the •Lactotriaosylceramide (Lc3Cer: GlcNAcβ1-3Galβ1-4GlcANcβ-Cer) synthase involved in the synthesis of neolacto-series glycolipids (involved in the synthesis of the polylactosamine on glycolipids). Furthermore, it has the activity to transfer GlcNAc also to the elongated paragloboside (paragloboside: nLc4Cer).

Keyword: Lactotriaosylceramide(Lc3Cer) , lacto-series glycolipid , neolacto-series glycolipid , poly-N-acetyllactosamine , polylactosamine

	UDP-GlcNAc
JCGG-STR001961 GlyTouCan: G84224TW GlyCosmos Glycans: G84224TW		JCGG-STR027022 GlyTouCan: G06239NA GlyCosmos Glycans: G06239NA

N-Acetylglucosaminyltransferase

GGDB Symbol

B3GNT5 Alias

Designation

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5

Organism

Homo sapiens

GeneID

84002

HGNC

B3GNT5 (15684)

mRNA

NM_032047

map

3q28

Protein

NP_114436

EC#

2.4.1.206

CAZy

GT31

GlyCosmos Glycogenes

B3GNT5

OMIM

615333

Disease name

GDGDB

Human Protein Atlas

ENSG00000176597

Mus musculus

mRNA	NM_054052
Protein	NP_473393
KO_mice_database	136
MGI	65235

Rattus norvegicus

mRNA	XM_344041
Protein	XP_344041

Glycobiology 2001-06-01 ;276(25):22032-40.

Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids.

Togayachi A. et al. PMID:11283017 Refer to: Table II,Table III,Figure 5

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR026439 GlyTouCan: G58896AZ GlyCosmos Glycans: G58896AZ	UDP-GlcNAc	JCGG-STR006200 GlyTouCan: G70874WW GlyCosmos Glycans: G70874WW

	JCGG-STR001961 GlyTouCan: G84224TW GlyCosmos Glycans: G84224TW	UDP-GlcNAc	JCGG-STR027022 GlyTouCan: G06239NA GlyCosmos Glycans: G06239NA

	JCGG-STR026439 GlyTouCan: G58896AZ GlyCosmos Glycans: G58896AZ	UDP-GlcNAc	JCGG-STR006200 GlyTouCan: G70874WW GlyCosmos Glycans: G70874WW

	JCGG-STR027036 GlyTouCan: G46055MA GlyCosmos Glycans: G46055MA	UDP-GlcNAc	JCGG-STR032528 GlyTouCan: G25181AD GlyCosmos Glycans: G25181AD

	JCGG-STR014685 GlyTouCan: G00058MO GlyCosmos Glycans: G00058MO	UDP-GlcNAc	JCGG-STR023826 GlyTouCan: G73480MV GlyCosmos Glycans: G73480MV

	JCGG-STR031350 GlyTouCan: G60687RJ GlyCosmos Glycans: G60687RJ	UDP-GlcNAc	JCGG-STR032531 GlyTouCan: G81934BB GlyCosmos Glycans: G81934BB

	JCGG-STR027037 GlyTouCan: G03362PH GlyCosmos Glycans: G03362PH	UDP-GlcNAc	JCGG-STR032529

Substrate Not Transferred

	JCGG-STR031555 GlyTouCan: G65889KE GlyCosmos Glycans: G65889KE

Glycobiology 2001-08-01 ;276(32):30261-9.

Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.

Henion TR. et al. PMID:11384981

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Glycobiology 2001-06-01 ;276(25):22032-40.

Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids.

Togayachi A. et al. PMID:11283017

Glycobiology 2001-08-01 ;276(32):30261-9.

Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.

Henion TR. et al. PMID:11384981

Glycobiology 2012-07-01 ;22(7):930-8.

High expression of lactotriaosylceramide, a differentiation-associated glycosphingolipid, in the bone marrow of acute myeloid leukemia patients.

Wang Z. et al. PMID:22411838

adrenal glandsamygdalabone marrowbraincaudate nucleuscerebellumcoloncorpus callosumfetal brainfetal kidneyfetal liverfetal lunghearthippocampuskidneyliverlunglymph nodemammary glandpancreaspituitary glandplacentaprostatesalivary glandskeletal musclesmall intestinespinal cordspleenstomachsubstantia nigratestisthalamusthymusthyroid glandtracheauterus

Provided by GOA

Function
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity
beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity
lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity

Process
O-glycan processing
brain development
central nervous system development
glycolipid biosynthetic process
protein glycosylation

Component
Golgi membrane
integral component of membrane
intracellular

B3GNT5

N-Acetylglucosaminyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Acceptor Substrates (Reference)

Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids.

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids.

Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.

High expression of lactotriaosylceramide, a differentiation-associated glycosphingolipid, in the bone marrow of acute myeloid leukemia patients.

Gene Ontology