GlycoProtDB ID - GPDB0005477

Protein Name Neprilysin
Protein Accession Number Q61391
Gene Mme
Length 750
Status
release : 2026-01-23

Glycosylation Sites
Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
MGRSESQMDI TDINAPKPKK KQRWTPLEIS LSVLVLLLTI IAVTMIALYA TYDDGICKSS DCIKSAARLI QNMDASVEPC TDFFKYACGG WLKRNVIPET
SSRYSNFDIL RDELEVILKD VLQEPKTEDI VAVQKAKTLY RSCINESAID SRGGQPLLKL LPDIYGWPVA SDNWDQTYGT SWTAEKSIAQ LNSKYGKKVL
INFFVGTDDK NSTQHIIHFD QPRLGLPSRD YYECTGIYKE ACTAYVDFMI SVARLIRQEQ SLPIDENQLS LEMNKVMELE KEIANATTKP EDRNDPMLLY
NKMTLAKLQN NFSLEVNGKS FSWSNFTNEI MSTVNINIQN EEEVVVYAPE YLTKLKPILT KYSPRDLQNL MSWRFIMDLV SSLSRNYKES RNAFRKALYG
TTSETATWRR CANYVNGNME NAVGRLYVEA AFAGESKHVV EDLIAQIREV FIQTLDDLTW MDAETKKKAE EKALAIKERI GYPDDIISNE NKLNNEYLEL
NYREDEYFEN IIQNLKFSQS KQLKKLREKV DKDEWISGAA VVNAFYSSGR NQIVFPAGIL QPPFFSAQQS NSLNYGGIGM VIGHEITHGF DDNGRNFNKD
GDLVDWWTQQ SANNFKDQSQ CMVYQYGNFS WDLAGGQHLN GINTLGENIA DNGGIGQAYR AYQNYVKKNG EEKLLPGLDL NHKQLFFLNF AQVWCGTYRP
EYAVNSIKTD VHSPGNFRII GTLQNSAEFA DAFHCRKNSY MNPERKCRVW

N-glycosylation sites
Potential sites Identified Peptide＊1
Position Sequon Position Sequence Unique or Shared
145 NES 142 - 152
SCINESAIDSR
Shared ( 5 )
211 NST 211 - 223
NSTQHIIHFDQPR
Shared ( 2 )
211 - 223
NSTQHIIHFDQPR
Shared ( 2 )
211 - 223
NSTQHIIHFDQPR
Shared ( 2 )
285 NAT 282 - 302
EIANATTKPEDRNDPMLLYNK
Unique
282 - 294
EIANATTKPEDRN
Unique
276 - 293
VMELEKEIANATTKPEDR
Unique
282 - 295
EIANATTKPEDRND
Unique
282 - 302
EIANATTKPEDRNDPMLLYNK
Unique
282 - 293
EIANATTKPEDR
Unique
282 - 302
EIANATTKPEDRNDPMLLYNK
Unique
282 - 302
EIANATTKPEDRNDPMLLYNK
Unique
311 NFS 308 - 319
LQNNFSLEVNGK
Unique
308 - 319
LQNNFSLEVNGK
Unique
308 - 319
LQNNFSLEVNGK
Unique
308 - 319
LQNNFSLEVNGK
Unique
325 NFT 320 - 354
SFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTK
Unique
628 NFS 617 - 660
DQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYR
Unique
Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Lung Pancreas Spleen Testis
Amide80 ConA RCA120 AAL AAL Amide80 RCA120 RCA120 ConA RCA120 Amide80 Amide80 Amide80 ConA RCA120
 
                           
   
 
   
   
                           
                           
     
                   
                           
   
           
 
   
                           
 
       
       
   
         
 
   
   
                   
                 
 
   
                           
                           
   
         
 
   
                           
                           
＊1:   _:Potential Sequon     ■:Asn (glycosylated)     ■:Gln (deaminated:pyroGlu)     ■:Met (oxidized)     ■:Cys (carbamidomethylated and deaminated)

External Links

Publications
Nagai-Okatani C, Tomioka A, Tominaga D, Sakaue H, Kuno A, Kaji H Inter-tissue glycan heterogeneity: site-specific glycoform analysis of mouse tissue N-glycoproteomes using MS1-based glycopeptide detection method assisted by lectin microarray Analytical and Bioanalytical Chemistry 2025;417(5):973-988 PMID:39676134
Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information
Entry version 2026-01-23
protein sequence version 2026-01-16
Entry status Latest version
Entry history

create : 2016-12-05

update : 2025-10-17

update : 2026-01-23