CHST2

Last Update:2021-12-10

CHST2 encodes carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, which has GlcNAc6ST enzymatic activity to transfer sulfate to the 6-position of GlcNAc at the non-reducing end. Five isoenzymes of GlcNAc6ST were found in human. CHST2 is involved in the biosynthesis of 6-sulfo sLeX, an L-selectin ligand, and in the biosynthesis of keratan sulfate.

Keyword: 6-sulfo sLeX , GlcNAc6ST , keratan sulfate , selectin ligand

	PAPS
JCGG-STR014102 GlyTouCan: G00065MO GlyCosmos Glycans: G00065MO		JCGG-STR023798 GlyTouCan: G99745QX GlyCosmos Glycans: G99745QX

	(not applicable)
JCGG-STR007591 GlyTouCan: G01187XC GlyCosmos Glycans: G01187XC		JCGG-STR009225 GlyTouCan: G51773AH GlyCosmos Glycans: G51773AH

Sulfotransferases

GGDB Symbol

CHST2 Alias

Designation

carbohydrate N-acetylglucosamine-6-O-sulfotransferase 2

Organism

Homo sapiens

GeneID

HGNC

CHST2 (1970)

mRNA

map

3q24

Protein

EC#

2.8.2.21

CAZy

GlyCosmos Glycogenes

CHST2

OMIM

603798

Disease name

GDGDB

Human Protein Atlas

ENSG00000175040

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Orthologous Gene

Mus musculus

mRNA	NM_018763
Protein	NP_061233
KO_mice_database	10
MGI	48541

Rattus norvegicus

mRNA	XP_345970
Protein	XP_345971

Acceptor Substrates (Reference)

J Biochem 1999-09-01 ;9(9):947-55.

Spatially and temporally regulated expression of N-acetylglucosamine-6-O-sulfotransferase during mouse embryogenesis.

Fan QW. et al. PMID:10460836

J Biochem 2000-10-01 ;1523(2-3):269-76.

Functional expression and genomic structure of human N-acetylglucosamine-6-O-sulfotransferase that transfers sulfate to beta-N-acetylglucosamine at the nonreducing end of an N-acetyllactosamine sequence.

Sakaguchi H. et al. PMID:11042394 Refer to: Table 1

DONOR : PAPS

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	PAPS	JCGG-STR008427 GlyTouCan: G17441VE GlyCosmos Glycans: G17441VE

	JCGG-STR023826 GlyTouCan: G73480MV GlyCosmos Glycans: G73480MV	PAPS	NO_ENTRY35

	JCGG-STR004754 GlyTouCan: G33476GF GlyCosmos Glycans: G33476GF	PAPS	JCGG-STR032537

Substrate Not Transferred

	JCGG-STR027487 GlyTouCan: G14371GV GlyCosmos Glycans: G14371GV

	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

	JCGG-STR007929 GlyTouCan: G13581JW GlyCosmos Glycans: G13581JW

	JCGG-STR017750 GlyTouCan: G40609EP GlyCosmos Glycans: G40609EP

	JCGG-STR007927 GlyTouCan: G95036CM GlyCosmos Glycans: G95036CM

J Biochem 1996-10-01 ;319 ( Pt 1)():209-16.

Characterization of a rat liver Golgi sulphotransferase responsible for the 6-O-sulphation of N-acetylglucosamine residues in beta-linkage to mannose: role in assembly of sialyl-galactosyl-N-acetylglucosamine 6-sulphate sequence of N-linked oligosaccharides.

Spiro RG. et al. PMID:8870671 Refer to: Table 1

DONOR : PAPS

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR023532 GlyTouCan: G14651SS GlyCosmos Glycans: G14651SS	PAPS	NO_ENTRY36

	JCGG-STR023199 GlyTouCan: G85856KC GlyCosmos Glycans: G85856KC	PAPS	NO_ENTRY37

	JCGG-STR004555 GlyTouCan: G48524PJ GlyCosmos Glycans: G48524PJ	PAPS	NO_ENTRY39

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	PAPS	JCGG-STR008427 GlyTouCan: G17441VE GlyCosmos Glycans: G17441VE

	JCGG-STR004754 GlyTouCan: G33476GF GlyCosmos Glycans: G33476GF	PAPS	JCGG-STR032537

	JCGG-STR014102 GlyTouCan: G00065MO GlyCosmos Glycans: G00065MO	PAPS	JCGG-STR023798 GlyTouCan: G99745QX GlyCosmos Glycans: G99745QX

Substrate Not Transferred

	JCGG-STR008690 GlyTouCan: G68544GH GlyCosmos Glycans: G68544GH

	JCGG-STR027022 GlyTouCan: G06239NA GlyCosmos Glycans: G06239NA

	JCGG-STR013995 GlyTouCan: G73578JC GlyCosmos Glycans: G73578JC

	JCGG-STR026960 GlyTouCan: G83513YI GlyCosmos Glycans: G83513YI

	JCGG-STR004787 GlyTouCan: G71661JQ GlyCosmos Glycans: G71661JQ

	JCGG-STR003580 GlyTouCan: G90562PB GlyCosmos Glycans: G90562PB

	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

J Biochem 1997-11-01 ;272(47):29493-501.

Characterization of an N-acetylglucosamine-6-O-sulfotransferase from human respiratory mucosa active on mucin carbohydrate chains.

Degroote S. et al. PMID:9368010 Refer to: FIG. 6.

DONOR : PAPS

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	PAPS	JCGG-STR008427 GlyTouCan: G17441VE GlyCosmos Glycans: G17441VE

	JCGG-STR004754 GlyTouCan: G33476GF GlyCosmos Glycans: G33476GF	PAPS	JCGG-STR032537

	JCGG-STR024754 GlyTouCan: G35679QA GlyCosmos Glycans: G35679QA	PAPS	JCGG-STR017706 GlyTouCan: G52120GQ GlyCosmos Glycans: G52120GQ

Substrate Not Transferred

	JCGG-STR026017 GlyTouCan: G18833GC GlyCosmos Glycans: G18833GC

	JCGG-STR008690 GlyTouCan: G68544GH GlyCosmos Glycans: G68544GH

	JCGG-STR022854 GlyTouCan: G08140FC GlyCosmos Glycans: G08140FC

	JCGG-STR001551 GlyTouCan: G91556EH GlyCosmos Glycans: G91556EH

	JCGG-STR030079 GlyTouCan: G04218XE GlyCosmos Glycans: G04218XE

J Biochem 1998-08-01 ;273(35):22577-83.

Molecular cloning and characterization of an N-acetylglucosamine-6-O-sulfotransferase.

Uchimura K. et al. PMID:9712885

DONOR : (not applicable)

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR007591 GlyTouCan: G01187XC GlyCosmos Glycans: G01187XC	(not applicable)	JCGG-STR009225 GlyTouCan: G51773AH GlyCosmos Glycans: G51773AH

J. Biol. Chem. 1998-09-01 ;124(3):670-8.

Human N-acetylglucosamine-6-O-sulfotransferase involved in the biosynthesis of 6-sulfo sialyl Lewis X: molecular cloning, chromosomal mapping, and expression in various organs and tumor cells.

Uchimura K. et al. PMID:9722682

Acceptor Substrates (KEM-C)

Expression

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Expression (Reference)

Biochim. Biophys. Acta 1999-02-01 ;55(3):345-7.

CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization.

Li X. et al. PMID:10049591

J. Biol. Chem. 1998-09-01 ;124(3):670-8.

Human N-acetylglucosamine-6-O-sulfotransferase involved in the biosynthesis of 6-sulfo sialyl Lewis X: molecular cloning, chromosomal mapping, and expression in various organs and tumor cells.

Uchimura K. et al. PMID:9722682

brainheartkidneyliverpancreasplacenta

Gene Ontology

Provided by GOA

Function
N-acetylglucosamine 6-O-sulfotransferase activity
sulfotransferase activity

Process
N-acetylglucosamine metabolic process
carbohydrate metabolic process
inflammatory response
keratan sulfate biosynthetic process
keratan sulfate synthesis
multicellular organism development
sulfur compound metabolic process

Component
Golgi membrane
integral component of membrane
intrinsic component of Golgi membrane
trans-Golgi network

This page does not indicate all of the enzymatic reaction, and expression of "CHST2".