GGDB:

CHST12は、carbohydrate (chondroitin 4) sulfotransferase 12をコードする。この酵素はGAGの1種であるコンドロイチン硫酸の生合成に関与する。コンドロイチン硫酸は（GalNAcβ1,4GlcAβ1,3）の2糖の繰り返し構造であるが、CHST12はGalNAcの4位に硫酸を転移するC4ST活性を有する。ヒトでは3種類のイソ酵素（CHST11, CHST12,CHST13）が存在する。CHST12はコンドロイチン硫酸のみならず、デルマタン硫酸（GalNAcβ1,4IdoAβ1,3）のIdoAに隣接したGalNAcの4位にも硫酸を転移する。

Keyword: コンドロイチン硫酸 , プロテオグリカン , 硫酸転移酵素

Sulfotransferases

GGDB Symbol

CHST12 Alias

Designation

chondroitin D-N-acetylgalactosamine-4-O-sulfotransferase 2

Organism

Homo sapiens

GeneID

55501

HGNC

CHST12 (17423)

mRNA

NM_018641

map

7p22

Protein

NP_061111

EC#

2.8.2.5

CAZy

GlyCosmos Glycogenes

CHST12

OMIM

610129

Disease name

GDGDB

Human Protein Atlas

ENSG00000136213

Mus musculus

mRNA	NM_021528
Protein	NP_067503
MGI	49454

Rattus norvegicus

mRNA	XM_221946
Protein	XP_221946

J. Biol. Chem. 2000-06-01 ;275(26):20188-96.

Molecular cloning and expression of two distinct human chondroitin 4-O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family.

Hiraoka N. et al. PMID:10781601

J. Biol. Chem. 2001-09-01 ;276(39):36344-53.

Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase.

Evers MR. et al. PMID:11470797

table 1
Glycoprotein	Enzyme activity
Dermatan (medium)	438
Chondroitin (medium)	352

J Biol Chem 2002-09-01 ;277(38):34766-72.

Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3. A novel member of the HNK-1 family of sulfotransferases.

Kang HG. et al. PMID:12080076 Refer to: TABLE I

DONOR : PAPS

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR029199 GlyTouCan: G71793UG GlyCosmos Glycans: G71793UG	PAPS	JCGG-STR029465 GlyTouCan: G00547ZN GlyCosmos Glycans: G00547ZN

table 1
Glycoprotein	Enzyme activity
Dermatan	127
Chondroitin	87

J Biol Chem 2003-09-01 ;278(38):36115-27.

Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor: implication of differential roles in dermatan sulfate biosynthesis.

Mikami T. et al. PMID:12847091

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

J. Biol. Chem. 2000-06-01 ;275(26):20188-96.

Molecular cloning and expression of two distinct human chondroitin 4-O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family.

Hiraoka N. et al. PMID:10781601

adrenal glandsfetal kidneyfetal lungfetal spleenheartlungperipheral blood leukocytepituitary glandsmall intestinespinal cordspleenthymusthyroid gland

Provided by GOA

Function
3'-phosphoadenosine 5'-phosphosulfate binding
chondroitin 4-sulfotransferase activity

Process
carbohydrate biosynthetic process
chondroitin sulfate biosynthetic process
dermatan sulfate biosynthetic process

Component
Golgi membrane
integral component of Golgi membrane
membrane

CHST12

Sulfotransferases

Orthologous Gene

Mus musculus

Rattus norvegicus

Acceptor Substrates (Reference)

Molecular cloning and expression of two distinct human chondroitin 4-O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family.

Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase.

Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3. A novel member of the HNK-1 family of sulfotransferases.

DONOR : PAPS

Substrate Transferred

Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor: implication of differential roles in dermatan sulfate biosynthesis.

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

Molecular cloning and expression of two distinct human chondroitin 4-O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family.

Gene Ontology