GGDB:

CHST9は、carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9をコードする。この酵素はN結合型糖鎖やO結合型糖鎖の非還元末端のGalNAcの4位に硫酸を転移する活性を有する。硫酸化GalNAcは、脳下垂体から分泌されるLHやTSHなどの糖タンパク質ホルモンに存在する特徴的な構造であり、ホルモンの血中からのクリアランスに機能する。ヒトでは2種類のイソ酵素（CHST8,CHST9）が存在し、発現組織やアクセプター基質特異性が異なる。

Keyword: LacdiNAc , 硫酸転移酵素 , 糖タンパク質ホルモン

Sulfotransferases

GGDB Symbol

CHST9 Alias

Designation

N-acetylgalactosamine-4-O-sulfotransferase

Organism

Homo sapiens

GeneID

83539

HGNC

CHST9 (19898)

mRNA

NM_031422

map

18q11.2

Protein

NP_113610

EC#

2.8.2.7

CAZy

GlyCosmos Glycogenes

CHST9

OMIM

610191

Disease name

GDGDB

Human Protein Atlas

ENSG00000154080

Mus musculus

mRNA	NM_199055
Protein	NP_951010
MGI	55347

Rattus norvegicus

mRNA	XM_226142
Protein	XP_226142

Glycobiology 2001-04-01 ;276(14):10861-9.

Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine.

Kang HG. et al. PMID:11139592 Refer to: TABLE I

DONOR : PAPS

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR029199 GlyTouCan: G71793UG GlyCosmos Glycans: G71793UG	PAPS	JCGG-STR029465 GlyTouCan: G00547ZN GlyCosmos Glycans: G00547ZN

	JCGG-STR029533 GlyTouCan: G00057MO GlyCosmos Glycans: G00057MO	PAPS	JCGG-STR007477 GlyTouCan: G33129LQ GlyCosmos Glycans: G33129LQ

Substrate Not Transferred

	JCGG-STR023199 GlyTouCan: G85856KC GlyCosmos Glycans: G85856KC

	JCGG-STR032552 GlyTouCan: G78126VZ GlyCosmos Glycans: G78126VZ

table 1
Glycoprotein	Enzyme activity
Chondroitin	36
Dermatan	21

J. Biol. Chem. 2001-06-01 ;11(6):495-504.

Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1-->4GlcNAc beta 1-->R in both N- and O-glycans.

Hiraoka N. et al. PMID:11445554

Glycobiology 2002-09-01 ;277(38):34766-72.

Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3. A novel member of the HNK-1 family of sulfotransferases.

Kang HG. et al. PMID:12080076 Refer to: TABLE I

DONOR : PAPS

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR029199 GlyTouCan: G71793UG GlyCosmos Glycans: G71793UG	PAPS	JCGG-STR029465 GlyTouCan: G00547ZN GlyCosmos Glycans: G00547ZN

table 1
Glycoprotein	Enzyme activity
Chondroitin	64
Dermatan	52

J. Biol. Chem. 2005-12-01 ;15(12):1349-58.

Differential expression and enzymatic properties of GalNAc-4-sulfotransferase-1 and GalNAc-4-sulfotransferase-2.

Boregowda RK. et al. PMID:16079414

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Glycobiology 2001-04-01 ;276(14):10861-9.

Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine.

Kang HG. et al. PMID:11139592

J. Biol. Chem. 2001-06-01 ;11(6):495-504.

Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1-->4GlcNAc beta 1-->R in both N- and O-glycans.

Hiraoka N. et al. PMID:11445554

J. Biol. Chem. 2005-12-01 ;15(12):1349-58.

Differential expression and enzymatic properties of GalNAc-4-sulfotransferase-1 and GalNAc-4-sulfotransferase-2.

Boregowda RK. et al. PMID:16079414

fetal lungheartliverpancreassalivary glandtestistrachea

Provided by GOA

Function
N-acetylgalactosamine 4-O-sulfotransferase activity
chondroitin 4-sulfotransferase activity

Process
carbohydrate biosynthetic process
chondroitin sulfate biosynthetic process
glycosaminoglycan metabolic process
hormone biosynthetic process
proteoglycan biosynthetic process
sulfur compound metabolic process

Component
Golgi membrane
extracellular region
integral component of membrane

CHST9

Sulfotransferases

Orthologous Gene

Mus musculus

Rattus norvegicus

Acceptor Substrates (Reference)

Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine.

DONOR : PAPS

Substrate Transferred

Substrate Not Transferred

Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1-->4GlcNAc beta 1-->R in both N- and O-glycans.

Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3. A novel member of the HNK-1 family of sulfotransferases.

DONOR : PAPS

Substrate Transferred

Differential expression and enzymatic properties of GalNAc-4-sulfotransferase-1 and GalNAc-4-sulfotransferase-2.

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine.

Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1-->4GlcNAc beta 1-->R in both N- and O-glycans.

Differential expression and enzymatic properties of GalNAc-4-sulfotransferase-1 and GalNAc-4-sulfotransferase-2.

Gene Ontology