GGDB:

ST8SIA4, ST8 α-N-acetylneuraminide α-2,8-sialyltransferase4, transfers sialic acid from CMP-sialic acid to the NeuAcα2→3-R and NeuAcα2→6-R structure on glycoproteins, mainly on the neural cell adhesion molecule (NCAM), synthesizing homopolymer of α2,8-linked sialic acid called polysialic acid. ST8SIA4 also synthesizes polysialic acid on O-glycans of Neuropilin-2 (NRP2). ST8SIA4 is also known as PST, SIAT8D, or ST8Sia-IV. Lack of ST8SIA4 impaired long-term potentiation and long-term depression in adult mice. Loss of polysialic acid by double knockout of ST8SIA2 and ST8SIA4 results in early neonatal lethal.

Keyword: NCAM , cell migration , neural development , polysialic acid

	CMP-Neu5Ac
JCGG-STR014102 GlyTouCan: G00065MO GlyCosmos Glycans: G00065MO		JCGG-STR033514

	CMP-Neu5Ac
JCGG-STR013995 GlyTouCan: G73578JC GlyCosmos Glycans: G73578JC		JCGG-STR033515

Sialyltransferase

GGDB Symbol

ST8SIA4 Alias

Designation

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4

Organism

Homo sapiens

GeneID

7903

HGNC

ST8SIA4 (10871)

mRNA

NM_005668

map

5q21

Protein

NP_005659

EC#

CAZy

GT29

GlyCosmos Glycogenes

ST8SIA4

OMIM

602547

Disease name

GDGDB

Human Protein Atlas

ENSG00000113532

Mus musculus

mRNA	NM_009183
Protein	NP_033209
MGI	26654

Rattus norvegicus

mRNA	U90215
Protein	AAB49989

J. Biochem. 2000-06-01 ;275(24):18594-601.

Differential biosynthesis of polysialic acid on neural cell adhesion molecule (NCAM) and oligosaccharide acceptors by three distinct alpha 2,8-sialyltransferases, ST8Sia IV (PST), ST8Sia II (STX), and ST8Sia III.

Angata K. et al. PMID:10766765

J. Biochem. 1995-09-01 ;118(3):658-64.

Molecular cloning and characterization of a third type of N-glycan alpha 2,8-sialyltransferase from mouse lung.

Yoshida Y. et al. PMID:8690732 Refer to: TABLE I.

DONOR : CMP-Neu5Ac

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR014102 GlyTouCan: G00065MO GlyCosmos Glycans: G00065MO	CMP-Neu5Ac	JCGG-STR033514

TABLE I. Comparison of the substrate specificities of three different N-glycan α2,8-sialyltransferases from mouse.
Acceptor	Enzyme activity (pmol/ml medium/h)
Fetuin	4.20
α1-Acid glycoprotein	3.30
Ovomucoid	0.26
Transferrin	0.20
Asialofetuin	0.00
BSM	0.00
Asialo-α1-acid glycoprotein	0.00

J. Biol. Chem. 1996-09-01 ;6(9):1188-91.

Polysialylation of NCAM by a single enzyme.

Mühlenhoff M. et al. PMID:8805371 Refer to: Figure 2

DONOR : CMP-Neu5Ac

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR013995 GlyTouCan: G73578JC GlyCosmos Glycans: G73578JC	CMP-Neu5Ac	JCGG-STR033515

J. Biochem. 1998-10-01 ;273(43):28524-32.

Differential and cooperative polysialylation of the neural cell adhesion molecule by two polysialyltransferases, PST and STX.

Angata K. et al. PMID:9774483

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Curr. Biol. 1995-07-01 ;92(15):7031-5.

Expression cloning of a human polysialyltransferase that forms the polysialylated neural cell adhesion molecule present in embryonic brain.

Nakayama J. et al. PMID:7624364

brainfetal brainfetal kidneyfetal lungheartlarge intestinelungperipheral blood leukocyteplacentasmall intestinespleenthymus

Provided by GOA

Function
alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity
sialic acid binding

Process
N-glycan processing
cellular protein modification process
ganglioside biosynthetic process
nervous system development
oligosaccharide metabolic process
protein glycosylation
sialylation

Component
Golgi membrane
integral component of Golgi membrane

ST8SIA4

Sialyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Acceptor Substrates (Reference)

Differential biosynthesis of polysialic acid on neural cell adhesion molecule (NCAM) and oligosaccharide acceptors by three distinct alpha 2,8-sialyltransferases, ST8Sia IV (PST), ST8Sia II (STX), and ST8Sia III.

Molecular cloning and characterization of a third type of N-glycan alpha 2,8-sialyltransferase from mouse lung.

DONOR : CMP-Neu5Ac

Substrate Transferred

Polysialylation of NCAM by a single enzyme.

DONOR : CMP-Neu5Ac

Substrate Transferred

Differential and cooperative polysialylation of the neural cell adhesion molecule by two polysialyltransferases, PST and STX.

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

Expression cloning of a human polysialyltransferase that forms the polysialylated neural cell adhesion molecule present in embryonic brain.

Gene Ontology