GGDB:

This gene encodes the enzyme that is involved in the synthesis of the Type 2 carbohydrate antigen (Galb1-4GlcNAc-R). This enzyme has the activity to transfer Gal from the donor UDP-Gal to the acceptor (GlcNAc-R) by the b1-4 bond. It is also known to be involved in the synthesis of the LACTOSE by binding to the α-lactalbumin and transferring the galactose to the glucose by using the UDP-galactose as a donor. On the other hand, it is also known that in the presence of the α-lactalbumin, the UDP-glucose is used as a donor and the glucose is also transferred to the N-acetylglucosamine.

Keyword: lactose , type II carbohydarte antigen , α-lactalbumin


JCGG-STR031465 GlyTouCan: G02522JF GlyCosmos Glycans: G02522JF

	UDP-Glc
JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO		JCGG-STR030022 GlyTouCan: G38884XS GlyCosmos Glycans: G38884XS

	UDP-Gal
JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO		JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Galactosyltransferase

GGDB Symbol

B4GALT1 Alias

Designation

beta 1,4-galactosyltransferase 1

Organism

Homo sapiens

GeneID

2683

HGNC

B4GALT1 (924)

mRNA

NM_001497

map

9p13

Protein

NP_001488

EC#

2.4.1.38

CAZy

GT31

GlyCosmos Glycogenes

B4GALT1

OMIM

137060

Disease name

CDGIId , Congenital Disorder of Glycosylation type IId

GDGDB

CON00361

Human Protein Atlas

ENSG00000086062

Mus musculus

mRNA	NM_022305
Protein	NP_071641
KO_mice_database	11
MGI	9081

Rattus norvegicus

mRNA	XM_342821
Protein	XP_342821

Nat. Methods 1999-12-01 ;1473(1):35-53.

Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions.

Amado M. et al. PMID:10580128

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR027022 GlyTouCan: G06239NA GlyCosmos Glycans: G06239NA	UDP-Gal	JCGG-STR026439 GlyTouCan: G58896AZ GlyCosmos Glycans: G58896AZ

	JCGG-STR006200 GlyTouCan: G70874WW GlyCosmos Glycans: G70874WW	UDP-Gal	JCGG-STR026436 GlyTouCan: G84034JH GlyCosmos Glycans: G84034JH

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Gal	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Nat. Methods 2001-10-01 ;276(40):37665-71.

alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc.

Ramakrishnan B. et al. PMID:11485999 Refer to: Table II,Table III

DONOR : UDP-Glc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Glc	JCGG-STR030022 GlyTouCan: G38884XS GlyCosmos Glycans: G38884XS

Nat. Methods 1970-08-01 ;9(5):297-300.

Purification of lactose synthetase a protein from human milk and demonstration of its interaction with alpha-lactalbumin.

Andrews P. et al. PMID:11947697

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR031569 GlyTouCan: G71142DF GlyCosmos Glycans: G71142DF	UDP-Gal	JCGG-STR001961 GlyTouCan: G84224TW GlyCosmos Glycans: G84224TW

Nat. Methods 2003-03-01 ;278(11):9150-8.

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

Seko A. et al. PMID:12511560

Nat. Methods 2007-07-01 ;4(7):577-82.

Strategy for the fine characterization of glycosyltransferase specificity using isotopomer assembly.

Ito H. et al. PMID:17529980

Nat. Methods 2008-04-01 ;9(4):292-309.

Structure and function of beta -1,4-galactosyltransferase.

Qasba PK. et al. PMID:18393823

Nat. Methods 1986-03-01 ;83(6):1573-7.

Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

Shaper NL. et al. PMID:2419911

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Gal	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Nat. Methods 1986-07-01 ;83(13):4720-4.

Cloning and sequencing of cDNA of bovine N-acetylglucosamine (beta 1-4)galactosyltransferase.

Narimatsu H. et al. PMID:3014508

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Gal	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Nat. Methods 1998-12-01 ;273(52):34843-9.

Synthesis of poly-N-acetyllactosamine in core 2 branched O-glycans. The requirement of novel beta-1,4-galactosyltransferase IV and beta-1,3-n-acetylglucosaminyltransferase.

Ujita M. et al. PMID:9857011 Refer to: TABLE I

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR027203 GlyTouCan: G82440PA GlyCosmos Glycans: G82440PA	UDP-Gal	JCGG-STR032532 GlyTouCan: G00887EN GlyCosmos Glycans: G00887EN

	JCGG-STR025658 GlyTouCan: G23000DQ GlyCosmos Glycans: G23000DQ	UDP-Gal	JCGG-STR027202 GlyTouCan: G80671XL GlyCosmos Glycans: G80671XL

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

		UDP-Gal
	JCGG-STR004435 GlyTouCan: G23438NR GlyCosmos Glycans: G23438NR		JCGG-STR031446 GlyTouCan: G07932PU GlyCosmos Glycans: G07932PU

		UDP-Gal
	JCGG-STR031467 GlyTouCan: G47557KS GlyCosmos Glycans: G47557KS		JCGG-STR031468 GlyTouCan: G68007WG GlyCosmos Glycans: G68007WG

		UDP-Gal
	JCGG-STR031449 GlyTouCan: G97494HI GlyCosmos Glycans: G97494HI		JCGG-STR004685 GlyTouCan: G86537AD GlyCosmos Glycans: G86537AD

		UDP-Gal
	JCGG-STR031450 GlyTouCan: G94541HG GlyCosmos Glycans: G94541HG		JCGG-STR031451 GlyTouCan: G32877EI GlyCosmos Glycans: G32877EI

		UDP-Gal
	JCGG-STR003666 GlyTouCan: G32426JY GlyCosmos Glycans: G32426JY		JCGG-STR003781 GlyTouCan: G94514IB GlyCosmos Glycans: G94514IB

		UDP-Gal
	JCGG-STR029201 GlyTouCan: G00033MO GlyCosmos Glycans: G00033MO		JCGG-STR025848 GlyTouCan: G64973KT GlyCosmos Glycans: G64973KT

		UDP-Gal
	JCGG-STR031447 GlyTouCan: G63334FZ GlyCosmos Glycans: G63334FZ		JCGG-STR031503 GlyTouCan: G25780HH GlyCosmos Glycans: G25780HH

		UDP-Gal
	JCGG-STR025794 GlyTouCan: G00035MO GlyCosmos Glycans: G00035MO		JCGG-STR027300 GlyTouCan: G32723SL GlyCosmos Glycans: G32723SL

		UDP-Gal
	JCGG-STR026235 GlyTouCan: G75472OU GlyCosmos Glycans: G75472OU		JCGG-STR031472 GlyTouCan: G77477NL GlyCosmos Glycans: G77477NL

		UDP-Gal
	JCGG-STR004668 GlyTouCan: G85608AG GlyCosmos Glycans: G85608AG		JCGG-STR027484 GlyTouCan: G46748BU GlyCosmos Glycans: G46748BU

Substrate Not Transferred


	JCGG-STR031465 GlyTouCan: G02522JF GlyCosmos Glycans: G02522JF


	JCGG-STR031464 GlyTouCan: G64844ET GlyCosmos Glycans: G64844ET


	JCGG-STR004795 GlyTouCan: G88696BY GlyCosmos Glycans: G88696BY


	JCGG-STR025848 GlyTouCan: G64973KT GlyCosmos Glycans: G64973KT


	JCGG-STR027483 GlyTouCan: G11457RF GlyCosmos Glycans: G11457RF


	JCGG-STR027221 GlyTouCan: G17752YB GlyCosmos Glycans: G17752YB


	JCGG-STR008757 GlyTouCan: G41245OZ GlyCosmos Glycans: G41245OZ


	JCGG-STR027300 GlyTouCan: G32723SL GlyCosmos Glycans: G32723SL

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Nat. Methods 2003-03-01 ;278(11):9150-8.

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

Seko A. et al. PMID:12511560

Nat. Methods 1998-05-01 ;8(5):517-26.

The expanding beta 4-galactosyltransferase gene family: messages from the databanks.

Lo NW. et al. PMID:9597550

adrenal glandsbone marrowheartkidneyliverlymph nodeovarypancreasperipheral blood leukocyteplacentaprostateskeletal musclespinal cordspleenstomachtestisthyroid glandtrachea

B4GALT1

Galactosyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Acceptor Substrates (Reference)

Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions.

DONOR : UDP-Gal

Substrate Transferred

alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc.

DONOR : UDP-Glc

Substrate Transferred

Purification of lactose synthetase a protein from human milk and demonstration of its interaction with alpha-lactalbumin.

DONOR : UDP-Gal

Substrate Transferred

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

Strategy for the fine characterization of glycosyltransferase specificity using isotopomer assembly.

Structure and function of beta -1,4-galactosyltransferase.

Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

DONOR : UDP-Gal

Substrate Transferred

Cloning and sequencing of cDNA of bovine N-acetylglucosamine (beta 1-4)galactosyltransferase.

DONOR : UDP-Gal

Substrate Transferred

Synthesis of poly-N-acetyllactosamine in core 2 branched O-glycans. The requirement of novel beta-1,4-galactosyltransferase IV and beta-1,3-n-acetylglucosaminyltransferase.

DONOR : UDP-Gal

Substrate Transferred

Acceptor Substrates (KEM-C)

DONOR : UDP-Gal

Substrate Transferred

Substrate Not Transferred

Expression

Expression (Reference)

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

The expanding beta 4-galactosyltransferase gene family: messages from the databanks.

Gene Ontology