GGDB:

本遺伝子は1型糖鎖抗原(Galb1-4GlcNAc-R)の合成に関与する酵素をコードしている。本酵素は糖供与体UDP-GalからGalをb1-4結合で糖受容体基質(GlcNAc-R)に転移する活性を有する。また、α−ラクトアルブミン(α-lactalbumin)と結合することで、UDP-galactoseを糖供与体として、ガラクトースをグルコースに転移し、LACTOSEの合成にも関与することが知られている。また一方、alpha-lactalbuminの存在下において、UDP-glucoseを糖供与体とし、グルコースをN−アセチルグルコサミンに転移する活性も有していることが知られている。

Keyword: 2型糖鎖抗原 , α−ラクトアルブミン , ラクトース


JCGG-STR031465 GlyTouCan: G02522JF GlyCosmos Glycans: G02522JF

	UDP-Glc
JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO		JCGG-STR030022 GlyTouCan: G38884XS GlyCosmos Glycans: G38884XS

	UDP-Gal
JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO		JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Galactosyltransferase

GGDB Symbol

B4GALT1 Alias

Designation

beta 1,4-galactosyltransferase 1

Organism

Homo sapiens

GeneID

2683

HGNC

B4GALT1 (924)

mRNA

NM_001497

map

9p13

Protein

NP_001488

EC#

2.4.1.90

CAZy

GT31

GlyCosmos Glycogenes

B4GALT1

OMIM

137060

Disease name

先天性糖鎖異常症IId型

GDGDB

CON00361

Human Protein Atlas

ENSG00000086062

Mus musculus

mRNA	NM_022305
Protein	NP_071641
KO_mice_database	11
MGI	9081

Rattus norvegicus

mRNA	XM_342821
Protein	XP_342821

Nat. Methods 1999-12-01 ;1473(1):35-53.

Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions.

Amado M. et al. PMID:10580128

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR027022 GlyTouCan: G06239NA GlyCosmos Glycans: G06239NA	UDP-Gal	JCGG-STR026439 GlyTouCan: G58896AZ GlyCosmos Glycans: G58896AZ

	JCGG-STR006200 GlyTouCan: G70874WW GlyCosmos Glycans: G70874WW	UDP-Gal	JCGG-STR026436 GlyTouCan: G84034JH GlyCosmos Glycans: G84034JH

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Gal	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Nat. Methods 2001-10-01 ;276(40):37665-71.

alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc.

Ramakrishnan B. et al. PMID:11485999 Refer to: Table II,Table III

DONOR : UDP-Glc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Glc	JCGG-STR030022 GlyTouCan: G38884XS GlyCosmos Glycans: G38884XS

Nat. Methods 1970-08-01 ;9(5):297-300.

Purification of lactose synthetase a protein from human milk and demonstration of its interaction with alpha-lactalbumin.

Andrews P. et al. PMID:11947697

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR031569 GlyTouCan: G71142DF GlyCosmos Glycans: G71142DF	UDP-Gal	JCGG-STR001961 GlyTouCan: G84224TW GlyCosmos Glycans: G84224TW

Biochim. Biophys. Acta 2003-03-01 ;278(11):9150-8.

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

Seko A. et al. PMID:12511560

Nat. Methods 2007-07-01 ;4(7):577-82.

Strategy for the fine characterization of glycosyltransferase specificity using isotopomer assembly.

Ito H. et al. PMID:17529980

Nat. Methods 2008-04-01 ;9(4):292-309.

Structure and function of beta -1,4-galactosyltransferase.

Qasba PK. et al. PMID:18393823

Nat. Methods 1986-03-01 ;83(6):1573-7.

Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

Shaper NL. et al. PMID:2419911

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Gal	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Nat. Methods 1986-07-01 ;83(13):4720-4.

Cloning and sequencing of cDNA of bovine N-acetylglucosamine (beta 1-4)galactosyltransferase.

Narimatsu H. et al. PMID:3014508

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO	UDP-Gal	JCGG-STR013782 GlyTouCan: G00055MO GlyCosmos Glycans: G00055MO

Nat. Methods 1998-12-01 ;273(52):34843-9.

Synthesis of poly-N-acetyllactosamine in core 2 branched O-glycans. The requirement of novel beta-1,4-galactosyltransferase IV and beta-1,3-n-acetylglucosaminyltransferase.

Ujita M. et al. PMID:9857011 Refer to: TABLE I

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR027203 GlyTouCan: G82440PA GlyCosmos Glycans: G82440PA	UDP-Gal	JCGG-STR032532 GlyTouCan: G00887EN GlyCosmos Glycans: G00887EN

	JCGG-STR025658 GlyTouCan: G23000DQ GlyCosmos Glycans: G23000DQ	UDP-Gal	JCGG-STR027202 GlyTouCan: G80671XL GlyCosmos Glycans: G80671XL

DONOR : UDP-Gal

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

		UDP-Gal
	JCGG-STR004435 GlyTouCan: G23438NR GlyCosmos Glycans: G23438NR		JCGG-STR031446 GlyTouCan: G07932PU GlyCosmos Glycans: G07932PU

		UDP-Gal
	JCGG-STR031467 GlyTouCan: G47557KS GlyCosmos Glycans: G47557KS		JCGG-STR031468 GlyTouCan: G68007WG GlyCosmos Glycans: G68007WG

		UDP-Gal
	JCGG-STR031449 GlyTouCan: G97494HI GlyCosmos Glycans: G97494HI		JCGG-STR004685 GlyTouCan: G86537AD GlyCosmos Glycans: G86537AD

		UDP-Gal
	JCGG-STR031450 GlyTouCan: G94541HG GlyCosmos Glycans: G94541HG		JCGG-STR031451 GlyTouCan: G32877EI GlyCosmos Glycans: G32877EI

		UDP-Gal
	JCGG-STR003666 GlyTouCan: G32426JY GlyCosmos Glycans: G32426JY		JCGG-STR003781 GlyTouCan: G94514IB GlyCosmos Glycans: G94514IB

		UDP-Gal
	JCGG-STR029201 GlyTouCan: G00033MO GlyCosmos Glycans: G00033MO		JCGG-STR025848 GlyTouCan: G64973KT GlyCosmos Glycans: G64973KT

		UDP-Gal
	JCGG-STR031447 GlyTouCan: G63334FZ GlyCosmos Glycans: G63334FZ		JCGG-STR031503 GlyTouCan: G25780HH GlyCosmos Glycans: G25780HH

		UDP-Gal
	JCGG-STR025794 GlyTouCan: G00035MO GlyCosmos Glycans: G00035MO		JCGG-STR027300 GlyTouCan: G32723SL GlyCosmos Glycans: G32723SL

		UDP-Gal
	JCGG-STR026235 GlyTouCan: G75472OU GlyCosmos Glycans: G75472OU		JCGG-STR031472 GlyTouCan: G77477NL GlyCosmos Glycans: G77477NL

		UDP-Gal
	JCGG-STR004668 GlyTouCan: G85608AG GlyCosmos Glycans: G85608AG		JCGG-STR027484 GlyTouCan: G46748BU GlyCosmos Glycans: G46748BU

Substrate Not Transferred


	JCGG-STR031465 GlyTouCan: G02522JF GlyCosmos Glycans: G02522JF


	JCGG-STR031464 GlyTouCan: G64844ET GlyCosmos Glycans: G64844ET


	JCGG-STR004795 GlyTouCan: G88696BY GlyCosmos Glycans: G88696BY


	JCGG-STR025848 GlyTouCan: G64973KT GlyCosmos Glycans: G64973KT


	JCGG-STR027483 GlyTouCan: G11457RF GlyCosmos Glycans: G11457RF


	JCGG-STR027221 GlyTouCan: G17752YB GlyCosmos Glycans: G17752YB


	JCGG-STR008757 GlyTouCan: G41245OZ GlyCosmos Glycans: G41245OZ


	JCGG-STR027300 GlyTouCan: G32723SL GlyCosmos Glycans: G32723SL

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Biochim. Biophys. Acta 2003-03-01 ;278(11):9150-8.

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

Seko A. et al. PMID:12511560

J. Biol. Chem. 1998-05-01 ;8(5):517-26.

The expanding beta 4-galactosyltransferase gene family: messages from the databanks.

Lo NW. et al. PMID:9597550

adrenal glandsbone marrowheartkidneyliverlymph nodeovarypancreasperipheral blood leukocyteplacentaprostateskeletal musclespinal cordspleenstomachtestisthyroid glandtrachea

B4GALT1

Galactosyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Acceptor Substrates (Reference)

Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions.

DONOR : UDP-Gal

Substrate Transferred

alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc.

DONOR : UDP-Glc

Substrate Transferred

Purification of lactose synthetase a protein from human milk and demonstration of its interaction with alpha-lactalbumin.

DONOR : UDP-Gal

Substrate Transferred

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

Strategy for the fine characterization of glycosyltransferase specificity using isotopomer assembly.

Structure and function of beta -1,4-galactosyltransferase.

Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

DONOR : UDP-Gal

Substrate Transferred

Cloning and sequencing of cDNA of bovine N-acetylglucosamine (beta 1-4)galactosyltransferase.

DONOR : UDP-Gal

Substrate Transferred

Synthesis of poly-N-acetyllactosamine in core 2 branched O-glycans. The requirement of novel beta-1,4-galactosyltransferase IV and beta-1,3-n-acetylglucosaminyltransferase.

DONOR : UDP-Gal

Substrate Transferred

Acceptor Substrates (KEM-C)

DONOR : UDP-Gal

Substrate Transferred

Substrate Not Transferred

Expression

Expression (Reference)

Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X.

The expanding beta 4-galactosyltransferase gene family: messages from the databanks.

Gene Ontology