GGDB:

GCNT1は、GlcNAcをUDP-GlcNAcからコア1型O-glycan structureのC6位のGalNAc, Galβ1→3GalNAc-Ser/Thrに転移しコア2型Galβ1→3(GlcNAcβ1→6)GalNAc-Ser/Thrを生成するコア２型グルコサミニル（Nアセチル）トランスフェラーゼ１である。GCNT1はコア2型β1,6 NアセチルグルコサミニルトランスフェラーゼI, C2GNT1あるいはC2GNT-Lとしても知られる。コア2型Oグリカンはムチン型Oグリカンの重要な構造の一つである。休止T細胞におけるGCNT1の過剰発現はT-B細胞相互作用に影響し免疫反応を減退させる。GCNT1の欠損で、GCNT1が白血球ローリングに部分的に関わっていることが示された。

Keyword: Oグリカン , コア2 , ムチン

	UDP-GlcNAc
JCGG-STR025711 GlyTouCan: G00031MO GlyCosmos Glycans: G00031MO		JCGG-STR029201 GlyTouCan: G00033MO GlyCosmos Glycans: G00033MO

N-Acetylglucosaminyltransferase

GGDB Symbol

GCNT1 Alias

Designation

glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)

Organism

Homo sapiens

GeneID

2650

HGNC

GCNT1 (4203)

mRNA

NM_001490

map

9q13

Protein

NP_001481

EC#

2.4.1.102

CAZy

GT14

GlyCosmos Glycogenes

GCNT1

OMIM

600391

Disease name

GDGDB

Human Protein Atlas

ENSG00000187210

Mus musculus

mRNA	NM_010265
Protein	NP_034395
KO_mice_database	29
MGI	9036

Rattus norvegicus

mRNA	NM_022276
Protein	NP_071612

Proc. Natl. Acad. Sci. U.S.A. 1992-10-01 ;89(19):9326-330.

Expression cloning of a cDNA encoding UDP-GlcNAc:Gal beta 1-3-GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen.

Bierhuizen MF. et al. PMID:1329093 Refer to: Table 1.

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR025711 GlyTouCan: G00031MO GlyCosmos Glycans: G00031MO	UDP-GlcNAc	JCGG-STR029201 GlyTouCan: G00033MO GlyCosmos Glycans: G00033MO

Proc. Natl. Acad. Sci. U.S.A. 1999-01-01 ;274(5):3215-21.

Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.

Yeh JC. et al. PMID:9915862 Refer to: FIG. 4.

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR025711 GlyTouCan: G00031MO GlyCosmos Glycans: G00031MO	UDP-GlcNAc	JCGG-STR029201 GlyTouCan: G00033MO GlyCosmos Glycans: G00033MO

	JCGG-STR025794 GlyTouCan: G00035MO GlyCosmos Glycans: G00035MO	UDP-GlcNAc	JCGG-STR022149 GlyTouCan: G00037MO GlyCosmos Glycans: G00037MO

Substrate Not Transferred

	JCGG-STR027203 GlyTouCan: G82440PA GlyCosmos Glycans: G82440PA

	JCGG-STR032532 GlyTouCan: G00887EN GlyCosmos Glycans: G00887EN

J. Biol. Chem. 1999-02-01 ;274(8):4504-12.

Control of O-glycan branch formation. Molecular cloning of human cDNA encoding a novel beta1,6-N-acetylglucosaminyltransferase forming core 2 and core 4.

Schwientek T. et al. PMID:9988682 Refer to: TABLE I

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR025711 GlyTouCan: G00031MO GlyCosmos Glycans: G00031MO	UDP-GlcNAc	JCGG-STR029201 GlyTouCan: G00033MO GlyCosmos Glycans: G00033MO

	JCGG-STR025711 GlyTouCan: G00031MO GlyCosmos Glycans: G00031MO	UDP-GlcNAc	JCGG-STR029201 GlyTouCan: G00033MO GlyCosmos Glycans: G00033MO

Substrate Not Transferred

	JCGG-STR025794 GlyTouCan: G00035MO GlyCosmos Glycans: G00035MO

	JCGG-STR025718 GlyTouCan: G57321FI GlyCosmos Glycans: G57321FI

	JCGG-STR025718 GlyTouCan: G57321FI GlyCosmos Glycans: G57321FI

	JCGG-STR026439 GlyTouCan: G58896AZ GlyCosmos Glycans: G58896AZ

	JCGG-STR004754 GlyTouCan: G33476GF GlyCosmos Glycans: G33476GF

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Proc. Natl. Acad. Sci. U.S.A. 1999-01-01 ;274(5):3215-21.

Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.

Yeh JC. et al. PMID:9915862

bone marrowbraincolonheartlunglymph nodeovarypancreasperipheral blood leukocyteplacentaprostateskeletal musclesmall intestinespleenstomachtestisthymusthyroid glandtrachea

Provided by GOA

Function
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity
protein binding

Process
O-glycan processing
cell adhesion molecule production
glycoprotein biosynthetic process
kidney morphogenesis
leukocyte tethering or rolling
response to insulin
tissue morphogenesis

Component
Golgi cisterna
Golgi membrane
extracellular space
integral component of membrane
trans-Golgi network

GCNT1

N-Acetylglucosaminyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Acceptor Substrates (Reference)

Expression cloning of a cDNA encoding UDP-GlcNAc:Gal beta 1-3-GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen.

DONOR : UDP-GlcNAc

Substrate Transferred

Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

Control of O-glycan branch formation. Molecular cloning of human cDNA encoding a novel beta1,6-N-acetylglucosaminyltransferase forming core 2 and core 4.

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.

Gene Ontology