GGDB:

β1,4-N-Acetylglucosaminyltransferase-IV (MGAT4) transfers GlcNAc toβ1-4 of mannose on the GlcNAcβ1-2Manα1-3 structure (GlcNAcβ1-2Manα1-3Manβ1-R) synthesized by MGAT1. MGAT4 does not have activity or affinity to the glycan structure synthesized by MGAT3 by receiving bisect GlcNac. MGAT4A, which has high homology to bovine enzymes, and MGAT4B, which has low homology to bovine enzymes, are known as MGAT4. Both are characterized as similar optimal pH and Mn2+ concentration, but are different in cation dependency. Co2+ can activate MGAT4B comparably to that Mn2+ does.

Keyword: GNT-IV , GNT-IVA

	UDP-GlcNAc
JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA		JCGG-STR006994 GlyTouCan: G20030CU GlyCosmos Glycans: G20030CU

	UDP-GlcNAc
JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ		JCGG-STR006972 GlyTouCan: G29011JC GlyCosmos Glycans: G29011JC

N-Acetylglucosaminyltransferase

GGDB Symbol

MGAT4A Alias

Designation

mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A

Organism

Homo sapiens

GeneID

11320

HGNC

MGAT4A (7047)

mRNA

NM_012214

map

2q12

Protein

NP_036346

EC#

2.4.1.145

CAZy

GT54

GlyCosmos Glycogenes

MGAT4A

OMIM

604623

Disease name

GDGDB

Human Protein Atlas

ENSG00000071073

Mus musculus

mRNA	NM_173870
Protein	NP_776295
MGI	90726

Rattus norvegicus

mRNA	XM_218731
Protein	XP_218731

Drosophila melanogaster

mRNA	NM_140463
Protein	NP_648720

J. Biol. Chem. 1999-03-01 ;9(3):303-10.

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

Yoshida A. et al. PMID:10024668

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR006972 GlyTouCan: G29011JC GlyCosmos Glycans: G29011JC

J. Biol. Chem. 2006-11-01 ;23(7-8):473-80.

Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes.

Oguri S. et al. PMID:17006639 Refer to: Table 4

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA	UDP-GlcNAc	JCGG-STR006994 GlyTouCan: G20030CU GlyCosmos Glycans: G20030CU

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR006972 GlyTouCan: G29011JC GlyCosmos Glycans: G29011JC

	JCGG-STR017422 GlyTouCan: G80858MF GlyCosmos Glycans: G80858MF	UDP-GlcNAc	JCGG-STR005993 GlyTouCan: G61207RZ GlyCosmos Glycans: G61207RZ

	JCGG-STR009312 GlyTouCan: G61751GZ GlyCosmos Glycans: G61751GZ	UDP-GlcNAc	JCGG-STR004886 GlyTouCan: G92024SL GlyCosmos Glycans: G92024SL

	JCGG-STR009245 GlyTouCan: G89993FE GlyCosmos Glycans: G89993FE	UDP-GlcNAc	JCGG-STR004198 GlyTouCan: G43299SD GlyCosmos Glycans: G43299SD

	JCGG-STR017804 GlyTouCan: G10133VD GlyCosmos Glycans: G10133VD	UDP-GlcNAc	JCGG-STR004349 GlyTouCan: G92022ML GlyCosmos Glycans: G92022ML

Substrate Not Transferred

	JCGG-STR003889 GlyTouCan: G12045WP GlyCosmos Glycans: G12045WP

J. Biol. Chem. 1983-05-01 ;258(10):6162-73.

Control of glycoprotein synthesis.

Gleeson PA. et al. PMID:6222042 Refer to: TABLE III

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR006972 GlyTouCan: G29011JC GlyCosmos Glycans: G29011JC

	JCGG-STR017422 GlyTouCan: G80858MF GlyCosmos Glycans: G80858MF	UDP-GlcNAc	JCGG-STR005993 GlyTouCan: G61207RZ GlyCosmos Glycans: G61207RZ

	JCGG-STR020757 GlyTouCan: G27919IH GlyCosmos Glycans: G27919IH	UDP-GlcNAc	JCGG-STR003681 GlyTouCan: G03445UI GlyCosmos Glycans: G03445UI

Substrate Not Transferred

	JCGG-STR017411 GlyTouCan: G07483YN GlyCosmos Glycans: G07483YN

	JCGG-STR002707 GlyTouCan: G99858XP GlyCosmos Glycans: G99858XP

	JCGG-STR020895 GlyTouCan: G58667NI GlyCosmos Glycans: G58667NI

	JCGG-STR013729 GlyTouCan: G78059CC GlyCosmos Glycans: G78059CC

	JCGG-STR014874 GlyTouCan: G77252PU GlyCosmos Glycans: G77252PU

	JCGG-STR013751 GlyTouCan: G59471TH GlyCosmos Glycans: G59471TH

	JCGG-STR020897 GlyTouCan: G30159WR GlyCosmos Glycans: G30159WR

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

J. Biol. Chem. 1998-12-01 ;15(12):1115-23.

A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment.

Yoshida A. et al. PMID:10372966

adrenal glandsbone marrowbraincolonheartkidneyliverlunglymph nodeovarypancreasperipheral blood leukocyteplacentaprostateskeletal musclesmall intestinespinal cordspleenstomachtestisthymusthyroid glandtrachea

Provided by GOA

Function
alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity
metal ion binding

Process
N-glycan processing

Component
Golgi membrane
extracellular exosome
integral component of membrane

MGAT4A

N-Acetylglucosaminyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Drosophila melanogaster

Acceptor Substrates (Reference)

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

DONOR : UDP-GlcNAc

Substrate Transferred

Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes.

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

Control of glycoprotein synthesis.

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment.

Gene Ontology