GGDB:

β1,4-N-Acetylglucosaminyltransferase-IV (MGAT4) transfers GlcNAc toβ1-4 of mannose on the GlcNAcβ1-2Manα1-3 structure (GlcNAcβ1-2Manα1-3Manβ1-R) synthesized by MGAT1. MGAT4 does not have activity or affinity to the glycan structure received bisect GlcNac by MGAT3. MGAT4A, which has high homology to bovine enzymes, and MGAT4B, which has low homology to bovine enzymes, are known as MGAT4. MGAT4B was cloned from human genome. Both are characterized as similar optimal pH and Mn2+ concentration, but are different in cation dependency. Co2+ can activate MGAT4B comparably to that Mn2+ does.

Keyword: GNT-IV , GNT-IVB

	UDP-GlcNAc
JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA		JCGG-STR006994 GlyTouCan: G20030CU GlyCosmos Glycans: G20030CU

	UDP-GlcNAc
JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ		JCGG-STR006972 GlyTouCan: G29011JC GlyCosmos Glycans: G29011JC

N-Acetylglucosaminyltransferase

GGDB Symbol

MGAT4B Alias

Designation

mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B

Organism

Homo sapiens

GeneID

11282

HGNC

MGAT4B (7048)

mRNA

NM_014275

map

5q35

Protein

NP_055090

EC#

2.4.1.145

CAZy

GT54

GlyCosmos Glycogenes

MGAT4B

OMIM

604561

Disease name

GDGDB

Human Protein Atlas

ENSG00000161013

Mus musculus

mRNA	NM_145926
Protein	NP_666038
MGI	71414

Rattus norvegicus

mRNA	XM_220372
Protein	XP_220372

Drosophila melanogaster

mRNA	NM_140464
Protein	NP_648721

Glycobiology 1999-03-01 ;9(3):303-10.

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

Yoshida A. et al. PMID:10024668

Glycobiology 1998-12-01 ;15(12):1115-23.

A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment.

Yoshida A. et al. PMID:10372966

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009312 GlyTouCan: G61751GZ GlyCosmos Glycans: G61751GZ	UDP-GlcNAc	JCGG-STR004886 GlyTouCan: G92024SL GlyCosmos Glycans: G92024SL

Glycoconj. J. 2006-11-01 ;23(7-8):473-80.

Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes.

Oguri S. et al. PMID:17006639 Refer to: Table 4

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA	UDP-GlcNAc	JCGG-STR006994 GlyTouCan: G20030CU GlyCosmos Glycans: G20030CU

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR006972 GlyTouCan: G29011JC GlyCosmos Glycans: G29011JC

	JCGG-STR017422 GlyTouCan: G80858MF GlyCosmos Glycans: G80858MF	UDP-GlcNAc	JCGG-STR005993 GlyTouCan: G61207RZ GlyCosmos Glycans: G61207RZ

	JCGG-STR009312 GlyTouCan: G61751GZ GlyCosmos Glycans: G61751GZ	UDP-GlcNAc	JCGG-STR004886 GlyTouCan: G92024SL GlyCosmos Glycans: G92024SL

	JCGG-STR017804 GlyTouCan: G10133VD GlyCosmos Glycans: G10133VD	UDP-GlcNAc	JCGG-STR004349 GlyTouCan: G92022ML GlyCosmos Glycans: G92022ML

	JCGG-STR009245 GlyTouCan: G89993FE GlyCosmos Glycans: G89993FE	UDP-GlcNAc	JCGG-STR004198 GlyTouCan: G43299SD GlyCosmos Glycans: G43299SD

Substrate Not Transferred

	JCGG-STR003889 GlyTouCan: G12045WP GlyCosmos Glycans: G12045WP

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Glycobiology 1998-12-01 ;15(12):1115-23.

A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment.

Yoshida A. et al. PMID:10372966

adrenal glandsbone marrowbraincolonheartkidneyliverlunglymph nodeovarypancreasperipheral blood leukocyteplacentaprostateskeletal musclesmall intestinespinal cordspleenstomachtestisthymusthyroid glandtrachea

Provided by GOA

Function
alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity
metal ion binding

Process
N-glycan processing

Component
Golgi membrane
integral component of membrane

MGAT4B

N-Acetylglucosaminyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Drosophila melanogaster

Acceptor Substrates (Reference)

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment.

DONOR : UDP-GlcNAc

Substrate Transferred

Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes.

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment.

Gene Ontology