GGDB:

β1,4-N-Acetylglucosaminyltransferase-V (MGAT5)は、MGAT2により合成されたGlcNAcβ1-2Manα1-6構造(GlcNAcβ1-2Manα1-6Manβ1-R)のMannoseにβ1-6でGlcNAcを転移する酵素である。GnT-Vは大部分のヒトや齧歯目の組織で発現しているのに対して、GnT-Vbの発現は主に神経組織と精巣に限られる。GnT-Vbは、N-とO-グリカン両方に対して、GlcNAcβ1,6Manを合成することができる。

Keyword: GNT-V , GNT5

	UDP-GlcNAc
JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ		JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

N-Acetylglucosaminyltransferase

GGDB Symbol

MGAT5 Alias

Designation

mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase

Organism

Homo sapiens

GeneID

4249

HGNC

MGAT5 (7049)

mRNA

NM_002410

map

2q21

Protein

NP_002401

EC#

2.4.1.155

CAZy

GT18

GlyCosmos Glycogenes

MGAT5

OMIM

601774

Disease name

GDGDB

Human Protein Atlas

ENSG00000152127

Mus musculus

mRNA	NM_145128
Protein	NP_660110
MGI	32971

Rattus norvegicus

mRNA	NM_023095
Protein	NP_075583

Caenorhabditis elegans

mRNA	NM_059473
Protein	NP_491874

Biochem. Biophys. Res. Commun. 1999-03-01 ;9(3):303-10.

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

Yoshida A. et al. PMID:10024668

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

J. Biol. Chem. 2010-02-01 ;20(2):166-74.

Comparison of the substrate specificities and catalytic properties of the sister N-acetylglucosaminyltransferases, GnT-V and GnT-Vb (IX).

Alvarez-Manilla G. et al. PMID:19846580 Refer to: Table I.

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

Substrate Not Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO

	JCGG-STR023199 GlyTouCan: G85856KC GlyCosmos Glycans: G85856KC

	JCGG-STR023199 GlyTouCan: G85856KC GlyCosmos Glycans: G85856KC

Biochem. Biophys. Res. Commun. 1982-11-01 ;257(22):13421-7.

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

Cummings RD. et al. PMID:6216250

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

Glycobiology 1994-01-01 ;198(1):318-27.

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Saito H. et al. PMID:8292036

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

Biochem. Biophys. Res. Commun. 1982-11-01 ;257(22):13421-7.

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

Cummings RD. et al. PMID:6216250

Glycobiology 1994-01-01 ;198(1):318-27.

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Saito H. et al. PMID:8292036

fetal liver

Provided by GOA

Function
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity

Process
protein N-linked glycosylation

Component
Golgi membrane
extracellular exosome
integral component of membrane

MGAT5

N-Acetylglucosaminyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Caenorhabditis elegans

Acceptor Substrates (Reference)

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

DONOR : UDP-GlcNAc

Substrate Transferred

Comparison of the substrate specificities and catalytic properties of the sister N-acetylglucosaminyltransferases, GnT-V and GnT-Vb (IX).

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

DONOR : UDP-GlcNAc

Substrate Transferred

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Gene Ontology