GGDB:

β1,4-N-Acetylglucosaminyltransferase-V (MGAT5)は、MGAT2により合成されたGlcNAcβ1-2Manα1-6構造(GlcNAcβ1-2Manα1-6Manβ1-R)のMannoseにβ1-6でGlcNAcを転移する酵素である。GnT-Vは大部分のヒトや齧歯目の組織で発現しているのに対して、GnT-Vbの発現は主に神経組織と精巣に限られる。GnT-Vbは、N-とO-グリカン両方に対して、GlcNAcβ1,6Manを合成することができる。

Keyword: GNT-V , GNT5

	UDP-GlcNAc
JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ		JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

N-Acetylglucosaminyltransferase

GGDB Symbol

MGAT5 Alias

Designation

mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase

Organism

Homo sapiens

GeneID

4249

HGNC

MGAT5 (7049)

mRNA

NM_002410

map

2q21

Protein

NP_002401

EC#

2.4.1.155

CAZy

GT18

GlyCosmos Glycogenes

MGAT5

OMIM

601774

Disease name

GDGDB

Human Protein Atlas

ENSG00000152127

Mus musculus

mRNA	NM_145128
Protein	NP_660110
MGI	32971

Rattus norvegicus

mRNA	NM_023095
Protein	NP_075583

Caenorhabditis elegans

mRNA	NM_059473
Protein	NP_491874

Biochem. Biophys. Res. Commun. 1999-03-01 ;9(3):303-10.

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

Yoshida A. et al. PMID:10024668

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

J. Biol. Chem. 2010-02-01 ;20(2):166-74.

Comparison of the substrate specificities and catalytic properties of the sister N-acetylglucosaminyltransferases, GnT-V and GnT-Vb (IX).

Alvarez-Manilla G. et al. PMID:19846580 Refer to: Table I.

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

Substrate Not Transferred

	JCGG-STR008276 GlyTouCan: G49108TO GlyCosmos Glycans: G49108TO

	JCGG-STR023199 GlyTouCan: G85856KC GlyCosmos Glycans: G85856KC

	JCGG-STR023199 GlyTouCan: G85856KC GlyCosmos Glycans: G85856KC

J. Biol. Chem. 1982-11-01 ;257(22):13421-7.

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

Cummings RD. et al. PMID:6216250

DONOR : UDP-GlcNAc

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR009224 GlyTouCan: G39213VZ GlyCosmos Glycans: G39213VZ	UDP-GlcNAc	JCGG-STR009323 GlyTouCan: G10219AA GlyCosmos Glycans: G10219AA

J. Biol. Chem. 1994-01-01 ;198(1):318-27.

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Saito H. et al. PMID:8292036

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

J. Biol. Chem. 1982-11-01 ;257(22):13421-7.

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

Cummings RD. et al. PMID:6216250

J. Biol. Chem. 1994-01-01 ;198(1):318-27.

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Saito H. et al. PMID:8292036

fetal liver

Provided by GOA

Function
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity

Process
protein N-linked glycosylation

Component
Golgi membrane
extracellular exosome
integral component of membrane

MGAT5

N-Acetylglucosaminyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Caenorhabditis elegans

Acceptor Substrates (Reference)

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase.

DONOR : UDP-GlcNAc

Substrate Transferred

Comparison of the substrate specificities and catalytic properties of the sister N-acetylglucosaminyltransferases, GnT-V and GnT-Vb (IX).

DONOR : UDP-GlcNAc

Substrate Transferred

Substrate Not Transferred

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

DONOR : UDP-GlcNAc

Substrate Transferred

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.

Gene Ontology