GGDB:

CHPFは、chondroitin polymerizing factorをコードし、GAGの1種であるコンドロイチン硫酸の生合成に関与する。コンドロイチン硫酸は（GalNAcβ1,4GlcAβ1,3）の2糖の繰り返し構造であるが、CHPFはCHSY1, CSGlcAT, CHPF2と複合体を形成して、コンドロイチン鎖の伸張反応を担うと考えられている。CHPF単独ではほとんど酵素活性を有さないが、CHSYと供発現すると高値のコンドロイチン伸長活性（GalNAc転移酵素活性とGlcA転移酵素活性）を示す。また、別の論文では、CHPFは単独でもGalNAc転移酵素活性とGlcA転移酵素活性を示すことが報告されている。

Keyword: コンドロイチン , コンドロイチン硫酸 , プロテオグリカン

	UDP-GalNAc
JCGG-STR032618 GlyTouCan: G43702JT GlyCosmos Glycans: G43702JT		JCGG-STR032619

N-Acetylgalactosaminyltransferase Glucuronyltransferase

GGDB Symbol

CHPF Alias

Designation

chondroitin sulfate synthase 2, chondroitin polymerizing factor, glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 2

Organism

Homo sapiens

GeneID

79586

HGNC

CHPF (24291)

mRNA

NM_024536

map

2q35

Protein

NP_078812

EC#

CAZy

GT31

GlyCosmos Glycogenes

CHPF

OMIM

610405

Disease name

GDGDB

Human Protein Atlas

ENSG00000123989

Mus musculus

mRNA	XM_129886
Protein	XP_129886

Rattus norvegicus

mRNA	XM_237306
Protein	XP_237306

Caenorhabditis elegans

mRNA	NM_066533
Protein	NP_498934

Drosophila melanogaster

mRNA	NM_132625
Protein	NP_572853

J Biol Chem 2003-06-01 ;278(26):23666-71.

Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.

Kitagawa H. et al. PMID:12716890

J. Biol. Chem. 2003-08-01 ;278(32):30235-47.

Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities.

Yada T. et al. PMID:12761225 Refer to: TABLE I

DONOR : UDP-GalNAc UDP-GlcUA

Glc	Man	Gal	GlcNAc	GalNAc	Fuc	Xyl	Neu5Ac	Neu5Gc	GlcA	IdoA	GlcN

Substrate Transferred

	JCGG-STR032616	UDP-GlcUA	JCGG-STR032617

	JCGG-STR032609	UDP-GlcUA	JCGG-STR032610

	JCGG-STR032619	UDP-GlcUA	JCGG-STR032620

	JCGG-STR032618 GlyTouCan: G43702JT GlyCosmos Glycans: G43702JT	UDP-GalNAc	JCGG-STR032619

	JCGG-STR032608	UDP-GalNAc	JCGG-STR032609

	JCGG-STR032615	UDP-GalNAc	JCGG-STR032616

	JCGG-STR032570 GlyTouCan: G70907ST GlyCosmos Glycans: G70907ST	UDP-GlcUA	JCGG-STR032621 GlyTouCan: G33235EO GlyCosmos Glycans: G33235EO

	JCGG-STR032569 GlyTouCan: G76533UX GlyCosmos Glycans: G76533UX	UDP-GalNAc	JCGG-STR032570 GlyTouCan: G70907ST GlyCosmos Glycans: G70907ST

	JCGG-STR032613 GlyTouCan: G03536SO GlyCosmos Glycans: G03536SO	UDP-GalNAc	JCGG-STR032614

	JCGG-STR032623 GlyTouCan: G19059PI GlyCosmos Glycans: G19059PI	UDP-GalNAc	JCGG-STR032534

	JCGG-STR032561 GlyTouCan: G34992DF GlyCosmos Glycans: G34992DF	UDP-GalNAc	JCGG-STR032612

	JCGG-STR032534	UDP-GlcUA	JCGG-STR032622

	JCGG-STR032624 GlyTouCan: G46732TY GlyCosmos Glycans: G46732TY	UDP-GalNAc	JCGG-STR032611

Substrate Not Transferred

	JCGG-STR032607 GlyTouCan: G34103VG GlyCosmos Glycans: G34103VG

	JCGG-STR032627 GlyTouCan: G16235VG GlyCosmos Glycans: G16235VG

	GAG_Hspoly

	GAG_heparin

	JCGG-STR032570 GlyTouCan: G70907ST GlyCosmos Glycans: G70907ST

	JCGG-STR032609

	JCGG-STR032616

	JCGG-STR032626 GlyTouCan: G86399RV GlyCosmos Glycans: G86399RV

	JCGG-STR032625 GlyTouCan: G60511GB GlyCosmos Glycans: G60511GB

	JCGG-STR027487 GlyTouCan: G14371GV GlyCosmos Glycans: G14371GV

	JCGG-STR032612

	JCGG-STR032611

	JCGG-STR032613 GlyTouCan: G03536SO GlyCosmos Glycans: G03536SO

	JCGG-STR032627 GlyTouCan: G16235VG GlyCosmos Glycans: G16235VG

	GAG_HSpoly

	GAG_heparin

	JCGG-STR032626 GlyTouCan: G86399RV GlyCosmos Glycans: G86399RV

	JCGG-STR032569 GlyTouCan: G76533UX GlyCosmos Glycans: G76533UX

	JCGG-STR032608

	JCGG-STR032615

	JCGG-STR032625 GlyTouCan: G60511GB GlyCosmos Glycans: G60511GB

	JCGG-STR028206 GlyTouCan: G87877ES GlyCosmos Glycans: G87877ES

	GAG_Nheparo_C1

	GAG_Nheparo

RNA Expression from Human Protein Atlas

This RNA expression data uses "HPA data" from the Human Protein Atlas. Tissue RNA Expression

data available from v22.proteinatlas.org

J Biol Chem 2003-06-01 ;278(26):23666-71.

Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.

Kitagawa H. et al. PMID:12716890

J. Biol. Chem. 2003-08-01 ;278(32):30235-47.

Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities.

Yada T. et al. PMID:12761225

adrenal glandsbone marrowbraincerebellumcerebral cortexcolonheartliverlungmammary glandovarypancreasperipheral blood leukocyteplacentaskeletal musclesmall intestinestomachtestisthymusthyroid glanduterus

CHPF

N-Acetylgalactosaminyltransferase Glucuronyltransferase

Orthologous Gene

Mus musculus

Rattus norvegicus

Caenorhabditis elegans

Drosophila melanogaster

Acceptor Substrates (Reference)

Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.

Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities.

DONOR : UDP-GalNAc UDP-GlcUA

Substrate Transferred

Substrate Not Transferred

Acceptor Substrates (KEM-C)

Expression

Expression (Reference)

Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.

Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities.

Gene Ontology