GlycoProtDB ID - GPDB0001128


Protein Name Autophagy-related protein 9A (Fragment)
Protein Accession Number A0A087WP33
Gene Atg9a
Length 847
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
XQRLEASYSD SPPGEEDLLV HVAEGSKSPW HHIENLDLFF SRVYNLHQKN GFTCMLIGEM FELMQFLFVV AFTTFLVSCV DYDILFANKM VNHSLHPTEP
VKVTLPDAFL PAQVCSARIQ ENGSLITILV IAGVFWIHRL IKFIYNICCY WEIHSFYLHA LRIPMSALPY CTWQEVQARI VQTQKEHQIC IHKRELTELD
IYHRILRFQN YMVALVNKSL LPLRFRLPGL GEVVFFTRGL KYNFELILFW GPGSLFLNEW SLKAEYKRGG QRLELAQRLS NRILWIGIAN FLLCPLILIW
QILYAFFSYA EVLKREPGAL GARCWSLYGR CYLRHFNELE HELQSRLNRG YKPASKYMNC FLSPLLTLLA KNGAFFAGSI LAVLIALTIY DEDVLAVEHV
LTTVTLLGVT VTVCRSFIPD QHMVFCPEQL LRVILAHIHY MPDHWQGNAH RSQTRDEFAQ LFQYKAVFIL EELLSPIVTP LILIFCLRPR ALEIIDFFRN
FT
VEVVGVGD TCSFAQMDVR QHGHPQWLSG GQTEASVYQQ AEDGKTELSL MHFAITNPGW QPPRESTAFL GFLKEQVQRD GAAAGLAQGG LLPENALFTS
IQSLQSESEP LSLIANVVAG SSCRGPSLSR DLQGSRHRAD VASALRSFSP LQPGAAPQGR VPSTMTGSGV DARTASSGSS VWEGQLQSLV LSEYASTEMS
LHALYMHQLH KQQTQAEPER HVWHRRESDE SGESAPEEGG EGARAPQPIP RSASYPCATP RPGAPETTAL HGGFQRRYGG ITDPGTVPRG PSHFSRLPLG
GWAEDGQPAS RHPEPVPEEG SEDELPPQVH KVKAVRPRTT ARSGDFQ

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
92 NHS 90 - 102
MVNHSLHPTEPVK
Shared ( 18 )
90 - 102
MVNHSLHPTEPVK
Shared ( 18 )
122 NGS
146 NIC
217 NKS
500 NFT
Heart Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Skeletal_muscle Stomach
ConA RCA120 AAL(+) ConA RCA120 WGA RCA120 RCA120 ConA ConA RCA120
   
   
 
     
                     
                     
                     
                     
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05