GlycoProtDB ID - GPDB0002057


Protein Name C4a protein
Protein Accession Number B9EIU2
Gene C4a
Length 1735
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
1001
1101
1201
1301
1401
1501
1601
1701
MWLLWGLAWV FSFCASSLQK PRLLLFSPSV VNLGTPLSVG VQLLDAPPGQ EVKGSVFLRN PKGGSCSPKK DFKLSSGDDF VLLSLEVPLE DVRSCGLFDL
RRAPYIQLVA QSPWLRNTAF KATETQGVNL LFSSRRGHIF VQTDQPIYNP GQRVRYRVFA LDQKMRPSTD FLTITVENSH GLRVLKKEIF TSTPILQDTF
IIPNISEPGT WKISARFSDG LESNRSTHFE VKKYVLPNFE VKITPWKPYI LMVPSNSDEI QLDIQARYIY GKPVQGVAYT RFALMDEQGK RTFLQGLETQ
AKLVEGRTHI SISKDQFQAA LDKINIGVRD LEGLHLYAAT AVIESPGGEM EEAELTSWRF VSSAFSLDLS RTKRHLVPGA HFLLQALVRE ISGSEASNVI
VKVSATLVSG SDSQVLNVQQ STNRIGQVSI SFPIPPTVTE LRLLVSAGSL YPTIARLTVQ SPPSRGTGFL SIEPLDPRSP RVGDTFILNL QAVGIPAPTF
SHYYYMIISR GQIMAMRREA RRTVTSVSVL VDHQLAPSFY FVAYFYHQGH PVANSLLINI QPRDCEGKLQ LKVVGAKEYR NGDMMKLQIQ TDSKALVALG
AVDTALYAVG GWSHKPLDMS KVFEVIDSYN LGCGPGGGDD APQVFQDAGL AFSDGDRLTQ TREDLSCPKE KKSRQKRNVN FLKALSEKLG QYSSPDAKCC
CQDGMAKLPM KRTCEQRAAR VPQQACREPF LSCCKFAEDL RRDQTRSQAP LARKVRDMVN LIEEDDILVR TSFPENWLWR VEPVDSSKLL TVRLPDSMTT
WEIHGVSLSK SKGLCVAKPT RVRVFRKFHL HLRLPISVRR FEQLELRPVL YNYLNDDKNK EGAIHREEIV YNLDPLNNLG QMLEIPGSSD PNIVPEGDFS
TFVKVTASEP LETLGSEGAL SPGGVASLLR LPRSCAEQTM IYLAPTLTAS NYLDRTKQWS KLSPETKDHA VHLIQKGHVR IQQFRKKDGS FGAWLHRDSS
TWLTAFVLKI LSLAQEQVGN FPEKLQETAS WLLAQQLGDG SFHDPCPVIH RAMQGGLVGS NETVALTAFV VIALHHGLNV FQEGHAKQLK NRVEASITKA
NSFLGQKASA GLLGAHAAAI TAYVLTLTKA SEDLRNVAHN SLMAMAEETG ENLYWGLVLG SQDKVVLRPA DPRSPTEPVP QAPALWIETT AYALLHLLLR
EGKGKMADKA ASWLTHQGNF HGAFRSTQDT VVTLDALSAY WIASHTTEEK VLKVTLSSMG RNGLKTHVLH LNNHQVKGLE EELKFSLGST ISVKVEGNSK
GTLKHGSEAN SLGKCAPSRM KWSQEDQWPK VIFAVPSVSP GYERSQEQAA FWQAGLSGRL HPSLQLHPTP CLILRTYNVL DMKNTTCQDL QIEVKVTDAV
EYAWSAYEDY EDDYNMPATD DPSVPLQPVT PLQLFEGRRS RRRREAPKVA EERESRVHYT VCIWRNGKLG LSGMAIEDIT LLSGFHALRA DLEKLTSLSD
RYVSHFETDG PHVLLYFDSV PTTRECVGFG ASQEVVVGLV QPASTVLYDY YSPDHKCSVF YAAPTKSQLL ATLCSGDVCQ CAEGKCPRLL RSLERRVEDK
DGYQMRFACY YHQVEYGFTV KVLREDGRAA FRLFESKITQ VLHFRTDTMA SIGQTRNFLS RASCRLRLEP NKEYLIMGMD GETSDNKGDP QYLLDSNTWI
EEMPSEQMCK STRHRAACFQ LKDFLMEFSS RGCQV

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
204 NIS
224 NRS 217 - 232
FSDGLESNRSTHFEVK
Shared ( 14 )
217 - 225
FSDGLESNR
Shared ( 14 )
217 - 233
FSDGLESNRSTHFEVKK
Shared ( 12 )
1061 NET
1384 NTT 1384 - 1395
NTTCQDLQIEVK
Shared ( 12 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(-/-) Lung Serum Skeletal_muscle Stomach Testis
ConA ConA RCA120 Amide80 ConA ConA AAL(+) AAL(+) ConA RCA120 ConA Amide80 ConA ConA ConA
                             
 
           
 
     
   
       
           
   
                             
           
     
 
 
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05