GlycoProtDB ID - GPDB0002665


Protein Name CD166 antigen (Fragment)
Protein Accession Number F6QH25
Gene Alcam
Length 345
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
XKLGDCISRD SYPDGNITWY RNGKVLQPVE GEVAILFKKE IDPGTQLYTV TSSLEYKTTR SDIQMPFTCS VTYYGPSGQK TIYSEQEIFD IYYLDLSLNP
SGEVTKQIGD TLPVSCTISA SRNATVVWMK DNIRLRSSPS FSSLHYQDAG NYVCETALQE VEGLKKRESL TLIVEGKPQI KMTKKTDPSG LSKTIICHVE
GFPKPAIHWT ITGSGSVINQ TEESPYINGR YYSKIIISPE ENVTLTCTAE NQLERTVNSL NVSAISIPEH DEADDISDEN REKVNDQAKL IVGIVVGLLL
AALVAGVVYW LYMKKSKTAS KHVNKDLGNM EENKKLEENN HKTEA

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
16 NIT 10 - 21
DSYPDGNITWYR
Shared ( 5 )
123 NAT 107 - 130
QIGDTLPVSCTISASRNATVVWMK
Shared ( 5 )
123 - 130
NATVVWMK
Shared ( 5 )
123 - 130
NATVVWMK
Shared ( 5 )
123 - 134
NATVVWMKDNIR
Shared ( 5 )
219 NQT
242 NVT 235 - 255
IIISPEENVTLTCTAENQLER
Shared ( 11 )
261 NVS 256 - 283
TVNSLNVSAISIPEHDEADDISDENREK
Shared ( 4 )
Brain Colon Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Stomach
RCA120 Amide80 AAL(+) AAL(+) AAL RCA120 RCA120 RCA120 ConA RCA120 Amide80 RCA120
 
 
   
 
                   
         
   
       
           
         
     
   
                       
 
     
 
 
       
   
 
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05