GlycoProtDB ID - GPDB0002897


Protein Name Apolipoprotein N, isoform CRA_a
Protein Accession Number G3X9D6
Gene Apon
Length 251
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
MIQAALLLGC ILLSPVTAFP WKTQNGSLPA VTRTEPTSNV LPNKIPPPTP ITCRDLLYTV LPAAPLSEFL SLLALRVVLE NIGCPAEAYS LQLRISEMGG
KDSAETLVLQ SQKSSQEEGI GNNEVILRHL VPSPGDMRRV RRSVTLPEAC TYEPGWVLYD MAQVLLETAN KLPPIDLVRE FKASVVNVTQ HCTMESWESM
NEVARRLMSS PELKDAKIPV EDRVYLVAQL AVVLKRIFVN LVWEYFQTYF G

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
25 NGS 23 - 33
TQNGSLPAVTR
Unique
187 NVT 183 - 205
ASVVNVTQHCTMESWESMNEVAR
Shared ( 2 )
183 - 205
ASVVNVTQHCTMESWESMNEVAR
Shared ( 2 )
Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Serum
ConA RCA120 RCA120 Amide80
 
   
     
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05