GlycoProtDB ID - GPDB0002941


Protein Name Alpha L integrin (Fragment)
Protein Accession Number H2ELL3
Gene Itgal
Length 1162
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
1001
1101
MSFRIAGPRL LLLGLQLFAK AWSYNLDTRP TQSFLAQAGR HFGYQVLQIE DGVVVGAPGE GDNTGGLYHC RTSSEFCQPV SLHGSNHTSK YLGMTLATDA
AKGSLLACDP GLSRTCDQNT YLSGLCYLFP QSLEGPMLQN RPAYQECMKG KVDLVFLFDG SQSLDRKDFE KILEFMKDVM RKLSNTSYQF AAVQFSTDCR
TEFTFLDYVK QNKNPDVLLG SVQPMFLLTN TFRAINYVVA HVFKEESGAR PDATKVLVII TDGEASDKGN ISAAHDITRY IIGIGKHFVS VQKQKTLHIF
ASEPVEEFVK ILDTFEKLKD LFTDLQRRIY AIEGTNRQDL TSFNMELSSS GISADLSKGH AVVGAVGAKD WAGGFLDLRE DLQGATFVGQ EPLTSDVRGG
YLGYTVAWMT SRSSRPLLAA GAPRYQHVGQ VLLFQAPEAG GRWNQTQKIE GTQIGSYFGG ELCSVDLDQD GEAELLLIGA PLFFGEQRGG RVFTYQRRQS
LFEMVSELQG DPGYPLGRFG AAITALTDIN GDRLTDVAVG APLEEQGAVY IFNGKPGGLS PQPSQRIQGA QVFPGIRWFG RSIHGVKDLG GDRLADVVVG
AEGRVVVLSS RPVVDVVTEL SFSPEEIPVH EVECSYSARE EQKHGVKLKA CFRIKPLTPQ FQGRLLANLS YTLQLDGHRM RSRGLFPDGS HELSGNTSIT
PDKSCLDFHF HFPICIQDLI SPINVSLNFS LLEEEGTPRD QKVGRAMQPI LRPSIHTVTK EIPFEKNCGE DKKCEANLTL SSPARSGPLR LMSSASLAVE
WTLSNSGEDA YWVRLDLDFP RGLSFRKVEM LQPHSRMPVS CEELTEGSSL LTKTLKCNVS SPIFKAGQEV SLQVMFNTLL NSSWEDFVEL NGTVHCENEN
SS
LQEDNSAA THIPVLYPVN ILTKEQENST LYISFTPKGP KTQQVQHVYQ VRIQPSAYDH NMPTLEALVG VPRPHSEDLI TYTWSVQTDP LVTCHSEDLK
RPSSEAEQPC LPGVQFRCPI VFRWEILIQV TGTVELSKEI KASSTLSLCS SLSVSFNSSK HFHLYGSKAS EAQVLVKVDL IHEKEMLHVY VLSGIGGLVL
LFLIFLALYK VGFFKRNLKE KMEADGGVPN GSPPEDTDPL AVPGEETKDM GCLEPLRESD KD

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
86 NHT 72 - 90
TSSEFCQPVSLHGSNHTSK
Shared ( 13 )
185 NTS
270 NIS 269 - 279
GNISAAHDITR
Shared ( 13 )
444 NQT 425 - 448
YQHVGQVLLFQAPEAGGRWNQTQK
Shared ( 14 )
668 NLS
696 NTS
724 NVS
728 NFS
777 NLT 773 - 785
KCEANLTLSSPAR
Shared ( 15 )
858 NVS 857 - 865
CNVSSPIFK
Shared ( 15 )
881 NSS
891 NGT
900 NSS
928 NST
1057 NSS
1130 NGS
Colon Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung
Amide80 AAL(+) AAL(+) ConA RCA120 RCA120 ConA
 
 
   
             
 
 
   
       
   
             
             
             
             
     
 
     
   
             
             
             
             
             
             
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05