GlycoProtDB ID - GPDB0003204


Protein Name Alpha-N-acetylglucosaminidase
Protein Accession Number O88325
Gene Naglu
Length 739
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
MEAAGLAVIL GFLLLAGGSV GDEAREAKAV RELVVRLLGP GPAANFLVSV ERALADESGL DTYSLSGGGG VPVLVRGSTG VAAAAGLHRY LRDFCGCQVA
WSSAQLHLPW PLPAVPDGLT ETTPNRYRYY QNVCTHSYSF VWWDWARWEQ EIDWMALNGI NLALAWNGQE AIWQRVYLAL GLTQSEIDTY FTGPAFLAWG
RMGNLHTWDG PLPRSWHLSQ VYLQHRILDR MRSFGMIPVL PAFAGHVPKA ITRVFPQVNV IKLGSWGHFN CSYSCSFLLA PGDPMFPLIG NLFLRELTKE
FGTDHIYGAD TFNEMQPPFS DPSYLAATTA AVYEAMVTVD PDAVWLLQGW LFQHQPQFWG PSQIRAVLEA VPRGRLLVLD LFAESHPVYM HTASFHGQPF
IWCMLHNFGG NHGLFGALED VNRGPQAARL FPNSTMVGTG IAPEGIGQNE VVYALMAELG WRKDPVPDLM AWVSSFAIRR YGVSQPDAVA AWKLLLRSVY
NCSGEACSGH NRSPLVKRPS LQMSTAVWYN RSDVFEAWRL LLTAAPNLTT SPAFRYDLLD VTRQAVQELV SLCYEEARTA YLKQELDLLL RAGGLLVYKL
LPTLDELLAS SSHFLLGTWL DQARKAAVSE AEAQFYEQNS RYQITLWGPE GNILDYANKQ LAGLVADYYQ PRWCLFLGTL AHSLARGVPF QQHEFEKNVF
PLEQAFVYNK KRYPSQPRGD TVDLSKKIFL KYHPQPDSL

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
132 NVC
270 NCS
433 NST
501 NCS 498 - 512
SVYNCSGEACSGHNR
Shared ( 2 )
498 - 517
SVYNCSGEACSGHNRSPLVK
Shared ( 2 )
511 NRS
530 NRS 518 - 531
RPSLQMSTAVWYNR
Shared ( 2 )
547 NLT 540 - 555
LLLTAAPNLTTSPAFR
Shared ( 2 )
540 - 563
LLLTAAPNLTTSPAFRYDLLDVTR
Shared ( 2 )
Brain Colon Heart Kidney Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Skeletal_muscle Stomach Testis
ConA Amide80 RCA120 ConA ConA RCA120 AAL ConA RCA120 SSA RCA120 RCA120 ConA ConA ConA RCA120 ConA RCA120
                                   
                                   
                                   
               
                 
                                   
           
 
               
       
 
 
     
             
                   
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05