GlycoProtDB ID - GPDB0003240


Protein Name Afamin
Protein Accession Number O89020
Gene Afm
Length 608
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
MRHLKLTGFI FFLLPLTESL ALPTKPQDVD HFNATQKFID ENTTYLAIIA FSQYVQEASF DEVETLVKVM LDYRDRCWAD NTLPECSKTA NDAIQDMLCD
MEGLPQKHNF SHCCGKAGFP RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA YKNNSESFLH LYMYEVARRN PFVFAPVLLA VAAWFEEAAT TCCEQQQKAT
CFQAKAAPIT QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK
VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK
FNETTQRSLA MVQQECKQFQ ELGKDTLQRH FLVKFTKAAP QLPMEELVSL SKEMVAALTT CCTLSDEFAC VDNLADLVLG ELCGVNTNRT INPAVDHCCK
TDFAFRRHCF EHLKADTTYE LPSVSALVSA LHTDWCQPRK EDLQNKKHRF LVNLVKWMPG ITDEEWLCLF TKFTAAREEC SEVQEPESCF SPESSKTGDE
SQATEKQR

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
33 NAT
42 NTT
109 NFS 89 - 116
TANDAIQDMLCDMEGLPQKHNFSHCCGK
Shared ( 3 )
89 - 116
TANDAIQDMLCDMEGLPQKHNFSHCCGK
Shared ( 3 )
89 - 116
TANDAIQDMLCDMEGLPQKHNFSHCCGK
Shared ( 3 )
153 NNS
222 NVC
338 NVC
402 NET 396 - 407
HVEDKFNETTQR
Shared ( 3 )
401 - 407
FNETTQR
Shared ( 3 )
488 NRT
Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Lung Serum Skeletal_muscle
Amide80 ConA ConA AAL(+) AAL(+) AAL ConA RCA120 ConA Amide80 Amide80
                     
                     
                 
 
                 
 
                 
 
                     
                     
                     
 
       
           
                     
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05