GlycoProtDB ID - GPDB0003332


Protein Name Cathepsin B
Protein Accession Number P10605
Gene Ctsb
Length 339
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
MWWSLILLSC LLALTSAHDK PSFHPLSDDL INYINKQNTT WQAGRNFYNV DISYLKKLCG TVLGGPKLPG RVAFGEDIDL PETFDAREQW SNCPTIGQIR
DQGSCGSCWA FGAVEAISDR TCIHTNGRVN VEVSAEDLLT CCGIQCGDGC NGGYPSGAWS FWTKKGLVSG GVYNSHVGCL PYTIPPCEHH VNGSRPPCTG
EGDTPRCNKS CEAGYSPSYK EDKHFGYTSY SVSNSVKEIM AEIYKNGPVE GAFTVFSDFL TYKSGVYKHE AGDMMGGHAI RILGWGVENG VPYWLAANSW
NLDWGDNGFF KILRGENHCG IESEIVAGIP RTDQYWGRF

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
38 NTT 37 - 45
QNTTWQAGR
Shared ( 3 )
37 - 45
QNTTWQAGR
Shared ( 3 )
192 NGS 165 - 206
KGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPR
Shared ( 2 )
166 - 206
GLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPR
Shared ( 2 )
185 - 206
PPCEHHVNGSRPPCTGEGDTPR
Shared ( 2 )
190 - 206
HVNGSRPPCTGEGDTPR
Shared ( 2 )
188 - 206
EHHVNGSRPPCTGEGDTPR
Shared ( 2 )
175 - 206
SHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPR
Shared ( 2 )
189 - 206
HHVNGSRPPCTGEGDTPR
Shared ( 2 )
208 NKS 207 - 223
CNKSCEAGYSPSYKEDK
Shared ( 4 )
317 NHC
Brain Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Lung Serum Skeletal_muscle Stomach Testis
ConA ConA ConA AAL(+) AAL(+) AAL ConA RCA120 ConA Amide80 Amide80 ConA ConA ConA
 
         
       
 
 
 
 
       
                 
       
 
 
 
   
 
     
                   
     
                 
     
                 
     
                   
     
                   
                 
       
                           
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05