GlycoProtDB ID - GPDB0003685


Protein Name CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
Protein Accession Number P54751
Gene St3gal1
Length 337
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
MRRKTLKYLT FFLLFIFLTS FVLNYSNTGV PSAWFPKQML LELSENFRRF IKSQPCTCRH CISQDKVSYW FDQRFNKTMQ PLLTVHNALM EEDTYRWWLR
LQRERKPNNL SDTVKELFRL VPGNVDPMLN KRLVGCRRCA VVGNSGNLKD SSYGPEIDSH DFVLRMNKAP TVGFEADVGS RTTHHLVYPE SFRELGENVN
MVLVPFKTTD LQWVISATTT GTITHTYVPV PPKIKVKQEK ILIYHPAFIK YVFDNWLQGH GRYPSTGILS IIFSIHICDE VDLYGFGADS KGNWHHYWEN
NPSAGAFRKT GVHDGDFEYN ITTTLAAINK IRIFKGR

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
24 NYS
76 NKT 67 - 77
VSYWFDQRFNK
Shared ( 4 )
109 NLS 104 - 115
ERKPNNLSDTVK
Shared ( 4 )
106 - 115
KPNNLSDTVK
Shared ( 4 )
320 NIT 310 - 330
TGVHDGDFEYNITTTLAAINK
Shared ( 4 )
Colon Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-)
Amide80 AAL RCA120 SSA RCA120 RCA120
           
       
   
 
   
   
 
   
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05