GlycoProtDB ID - GPDB0003758


Protein Name C-type lectin domain family 4 member F
Protein Accession Number P70194
Gene Clec4f
Length 548
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
MKEAELNRDM ARYCTDNQCV SLQPQGLGPK SAALMAPRTL RHVQVILALM VVTVIFSLLA LFVVASQPWR PEWNKEPPSL LLRGSNNSGH DNHSQFVRET
EMQVAIQRLR DYEENSSSCH KEVQILKYQM DNVSSLVQLL GSHLEDVNAD ILQTKDVLKE SGALALETQA LRSSLEVASA DIHSLRGDLE KANAMTSQTR
GLLKSSTENT SAELHVLGRG LEEAQSEIQA LRGSLQSAND LSSQTQGFLQ HSMDNISAQI QTVRDGMERA GEKMNSLKKE LETLTAQTQK ANGHLEQTDA
QIQGLKAELK STSSLNSRIE VVNGQMKDAS RELQTLRRDL SDVSALKSNV QMLQSNLQRA KTEMQTLKAD LQATKALTAK IQGEQNRLGA LQEAVAAQKQ
EQKTQNQVLQ LIAQNWKYFN GNFYYFSRDK KPWREAEKFC TSQGAHLASV TSQEEQAFLV QTTSSGDHWI GLTDQGTEGI WRWVDGTPFN NAQSKGFWGK
NQPDNWRHRN GEREDCVHVR QQWNDMACGS SYPWVCKKST GWSAARVG

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
17 NQC
86 NNS 84 - 98
GSNNSGHDNHSQFVR
Shared ( 2 )
84 - 98
GSNNSGHDNHSQFVR
Shared ( 2 )
84 - 108
GSNNSGHDNHSQFVRETEMQVAIQR
Shared ( 2 )
92 NHS 84 - 98
GSNNSGHDNHSQFVR
Shared ( 2 )
84 - 108
GSNNSGHDNHSQFVRETEMQVAIQR
Shared ( 2 )
115 NSS 109 - 121
LRDYEENSSSCHK
Shared ( 2 )
132 NVS 128 - 155
YQMDNVSSLVQLLGSHLEDVNADILQTK
Shared ( 2 )
128 - 155
YQMDNVSSLVQLLGSHLEDVNADILQTK
Shared ( 2 )
209 NTS 205 - 219
SSTENTSAELHVLGR
Shared ( 2 )
255 NIS 233 - 264
GSLQSANDLSSQTQGFLQHSMDNISAQIQTVR
Shared ( 2 )
Heart Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Serum
Amide80 AAL(+) AAL(+) AAL ConA RCA120 WGA RCA120 RCA120 Amide80
                   
     
 
 
         
   
 
             
   
     
 
 
             
   
 
               
 
   
 
 
   
     
 
     
 
 
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05