GlycoProtDB ID - GPDB0003812


Protein Name Aminopeptidase N
Protein Accession Number P97449
Gene Anpep
Length 966
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
MAKGFYISKT LGILGILLGV AAVCTIIALS VVYAQEKNRN AENSATAPTL PGSTSATTAT TTPAVDESKP WNQYRLPKTL IPDSYRVILR PYLTPNNQGL
YIFQGNSTVR FTCNQTTDVI IIHSKKLNYT LKGNHRVVLR TLDGTPAPNI DKTELVERTE YLVVHLQGSL VEGRQYEMDS QFQGELADDL AGFYRSEYME
GDVKKVVATT QMQAADARKS FPCFDEPAMK AMFNITLIYP NNLIALSNML PKESKPYPED PSCTMTEFHS TPKMSTYLLA YIVSEFKNIS SVSANGVQIG
IWARPSAIDE GQGDYALNVT GPILNFFAQH YNTSYPLPKS DQIALPDFNA GAMENWGLVT YRESSLVFDS QSSSISNKER VVTVIAHELA HQWFGNLVTV
AWWNDLWLNE GFASYVEYLG ADYAEPTWNL KDLMVLNDVY RVMAVDALAS SHPLSSPADE IKTPDQIMEL FDSITYSKGA SVIRMLSSFL TEDLFKKGLS
SYLHTYQYSN TVYLDLWEHL QKAVNQQTAV QPPATVRTIM DRWILQMGFP VITVNTNTGE ISQKHFLLDS KSNVTRPSEF NYIWIAPIPF LKSGQEDHYW
LDVEKNQSAK FQTSSNEWIL LNINVTGYYL VNYDENNWKK LQNQLQTDLS VIPVINRAQI IHDSFNLASA KMIPITLALD NTLFLVKEAE YMPWQAALSS
LNYFTLMFDR SEVYGPMKRY LKKQVTPLFF YFQNRTNNWV NRPPTLMEQY NEINAISTAC SSGLKECRDL VVELYSQWMK NPNNNTIHPN LRSTVYCNAI
AFGGEEEWNF AWEQFRNATL VNEADKLRSA LACSKDVWIL NRYLSYTLNP DYIRKQDTTS TIISIASNVA GHPLVWDFVR SNWKKLFENY GGGSFSFANL
IQGVTRRFSS EFELQQLEQF KADNSATGFG TGTRALEQAL EKTRANIDWV KENKDAVFKW FTENSS

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
106 NST 87 - 110
VILRPYLTPNNQGLYIFQGNSTVR
Shared ( 2 )
114 NQT 111 - 125
FTCNQTTDVIIIHSK
Shared ( 2 )
128 NYT 126 - 132
KLNYTLK
Shared ( 2 )
234 NIT
288 NIS
318 NVT
332 NTS
573 NVT 572 - 592
SNVTRPSEFNYIWIAPIPFLK
Shared ( 3 )
606 NQS 593 - 610
SGQEDHYWLDVEKNQSAK
Shared ( 3 )
624 NVT
734 NRT 729 - 735
FFYFQNR
Shared ( 3 )
724 - 735
QVTPLFFYFQNR
Shared ( 3 )
724 - 735
QVTPLFFYFQNR
Shared ( 3 )
784 NNT 769 - 792
DLVVELYSQWMKNPNNNTIHPNLR
Shared ( 4 )
781 - 792
NPNNNTIHPNLR
Shared ( 4 )
781 - 790
NPNNNTIHPN
Shared ( 4 )
817 NAT 817 - 828
NATLVNEADKLR
Shared ( 11 )
817 - 826
NATLVNEADK
Shared ( 4 )
964 NSS
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Serum Skeletal_muscle Stomach Testis
ConA RCA120 Amide80 ConA RCA120 Amide80 ConA RCA120 AAL ConA RCA120 AAL(+) AAL(+) AAL ConA RCA120 SSA WGA RCA120 RCA120 ConA RCA120 Amide80 Amide80 ConA RCA120 Amide80 ConA RCA120 RCA120
                     
 
                             
     
   
 
 
                 
                     
                                 
                                                           
                                                           
                                                           
                                                           
                     
         
               
 
               
     
 
           
             
                                                           
                     
                                 
   
 
 
 
   
 
 
 
 
 
 
 
   
 
 
 
 
   
     
 
   
 
                 
                                     
 
 
 
 
 
   
   
 
   
 
                     
                                   
 
               
     
                         
                     
         
                     
                                                           
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05