GlycoProtDB ID - GPDB0003977


Protein Name 5' nucleotidase, ecto
Protein Accession Number Q0VEE0
Gene Nt5e
Length 576
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
MRPAAAKVPK WLLLALSALL PQWPAASAWE LTILHTNDVH SRLEQTSDDS TKCLNASLCV GGVARLFTKV QQIRKEEPNV LFLDAGDQYQ GTIWFTVYKG
LEVAHFMNIL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG PLAHQISGLF LPSKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEIS
ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDI VVGGHSNTFL YTGNPPSKEV PAGKYPFIVT ADDGRQVPVV QAYAFGKYLG YLKVEFDDKG
NVITSYGNPI LLNSSIPEDA TIKADINQWR IKLDNYSTQE LGRTIVYLDG STQTCRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI
DEKNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE FLQVGGIHVV YDINRKPWNR VVQLEVLCTK CRVPIYEPLE MDKVYKVTLP
SYLANGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVV YPAVEGRIKF SAASHYQGSF PLVILSFWAM ILILYQ

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
55 NAS 53 - 65
CLNASLCVGGVAR
Shared ( 3 )
313 NSS 300 - 323
GNVITSYGNPILLNSSIPEDATIK
Shared ( 3 )
335 NYS 331 - 343
IKLDNYSTQELGR
Shared ( 3 )
333 - 343
LDNYSTQELGR
Shared ( 3 )
405 NGT 404 - 429
NNGTITWENLAAVLPFGGTFDLVQLK
Shared ( 2 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Stomach Testis
RCA120 Amide80 Amide80 AAL RCA120 AAL(+) AAL(+) RCA120 WGA RCA120 RCA120 ConA RCA120 Amide80 ConA RCA120
     
 
 
 
 
                 
           
   
   
 
   
 
 
     
 
 
                 
           
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05