GlycoProtDB ID - GPDB0004171


Protein Name Alpha-mannosidase (Fragment)
Protein Accession Number Q3TB95
Gene Man2b1
Length 516
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
QASLFAQMGF DGFFLGRIDY QDKLNRKKKL RMEELWRASD SLEPPAADLF TGVLPNNYNP PKYLCWDVLC TDPPVVDNPR SPEFNAKTLV NYFLKLASSQ
KGFYRTNHTV MTMGSDFHYE NANMWFKNMD KLIRLVNAQQ ANGSLVHVLY STPTCYLWEL NKANLTWTVK EDDFFPYADG PHMFWTGYFS SRPALKRYER
LSYNFLQVCN QLEALVGPEA NVGPYGSGDS APLQEAMAVL QHHDAVSGTA RQNVVNDYAR QLAAGWGPCE VLVSNALARL SHYKQNFSFC RELNISICPV
SQTSERFQVT LYNPLGRKVD QMVRLPVYEG NFIVKDPHDK NISSNVVMVP SYYSETYQWE LLFPASVPAL GFSTYSVAKM SDLNHQAHNL LSRPRKHKSH
HVLVIENKYM RATFDSGTGL LMKIENLEQN LSLPVSQGFF WYNASVGDEE SSQASGAYIF RPNVGKPIPV SRWAQISLVK TALVQEVHQN FSAWCSQVIR
LYKGQRHLEL EWTVGP

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
107 NHT 106 - 127
TNHTVMTMGSDFHYENANMWFK
Shared ( 3 )
142 NGS
164 NLT 163 - 170
ANLTWTVK
Shared ( 3 )
286 NFS 280 - 291
LSHYKQNFSFCR
Shared ( 3 )
285 - 291
QNFSFCR
Shared ( 3 )
285 - 306
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
285 - 306
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
294 NIS 292 - 306
ELNISICPVSQTSER
Shared ( 3 )
285 - 306
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
285 - 306
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
341 NIS
430 NLS
443 NAS
490 NFS 481 - 500
TALVQEVHQNFSAWCSQVIR
Shared ( 3 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Serum Skeletal_muscle Stomach Testis
ConA ConA RCA120 ConA AAL ConA AAL(+) AAL(+) AAL ConA RCA120 SSA RCA120 RCA120 AAL ConA Amide80 ConA ConA ConA RCA120
                       
               
                                         
     
     
 
 
     
 
               
 
       
       
     
     
                                   
   
                                   
   
 
     
         
 
 
 
                                   
   
                                   
   
                                         
                                         
                                         
       
     
 
 
     
 
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05