GlycoProtDB ID - GPDB0004176


Protein Name Alpha-mannosidase
Protein Accession Number Q3TBM1
Gene Man2b1
Length 1013
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
1001
MGTGPLTSGV RSGGGNTGWL WMSSCNLGSP VLPISFLFWL LLAAPGARAA GYKTCPPTKP GMLNVHLLPH THDDVGWLKT VDQYYYGILS DVQHASVQYI
LDSVVSSLLE KPTRRFIYVE MAFFSRWWKQ QTSATQDAVR NLVRQGRLEF VNGGWVMNDE AATHYGAIVD QMTLGLRFLQ DTFGSDGLPR VAWHIDPFGH
SREQASLFAQ MGFDGFFLGR IDYQDKLNRK KKLRMEELWR ASDSLEPPAA DLFTGVLPNN YNPPKYLCWD VLCTDPPVVD NPRSPEFNAK TLVNYFLKLA
SSQKGFYRTN HTVMTMGSDF HYENANMWFK NMDKLIRLVN AQQANGSLVH VLYSTPTCYL WELNKANLTW TVKEDDFFPY ADGPHMFWTG YFSSRPALKR
YERLSYNFLQ VCNQLEALVG PEANVGPYGS GDSAPLQEAM AVLQHHDAVS GTARQNVVND YARQLAAGWG PCEVLVSNAL ARLSHYKQNF SFCRELNISI
CPVSQTSERF QVTLYNPLGR KVDQMVRLPV YEGNFIVKDP HDKNISSNVV MVPSYYSETY QWELLFPASV PALGFSTYSV AKMSDLNHQA HNLLSRPRKH
KSHHVLVIEN KYMRATFDSG TGLLMKIENL EQNLSLPVSQ GFFWYNASVG DEESSQASGA YIFRPNVGKP IPVSRWAQIS LVKTALVQEV HQNFSAWCSQ
VIRLYKGQRH LELEWTVGPI PVRDDWGKEV ISRFDTPMKT KGQFFTDSNG REILKRRDDY RPTWTLNQTE PVAGNYYPVN TRIYITDGQM QLTVLTDRSQ
GGSSLQDGSL ELMVHRRLLV DDDRGVSEPL LETDTGDKVR GRHLVLLSSV SDAAARHRLL AEQEVLAPQV VLSLGGSSPY HSRATPKTQF SGLRQELPPQ
VHLLTLARWG PKMLLLRLEH QFALKEDSDR NLSSPVTLNV QNLFQTFTIN YLQETTLAAN QPLSRASRLK WMTNTGPTSF PEPSKLDPTS VTLKPMEIRT
FLASVQWQEH RPA

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
310 NHT 309 - 330
TNHTVMTMGSDFHYENANMWFK
Shared ( 3 )
345 NGS
367 NLT 366 - 373
ANLTWTVK
Shared ( 3 )
489 NFS 483 - 494
LSHYKQNFSFCR
Shared ( 3 )
488 - 494
QNFSFCR
Shared ( 3 )
488 - 509
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
488 - 509
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
497 NIS 495 - 509
ELNISICPVSQTSER
Shared ( 3 )
488 - 509
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
488 - 509
QNFSFCRELNISICPVSQTSER
Shared ( 3 )
544 NIS
633 NLS
646 NAS
693 NFS 684 - 703
TALVQEVHQNFSAWCSQVIR
Shared ( 3 )
767 NQT 756 - 782
RRDDYRPTWTLNQTEPVAGNYYPVNTR
Shared ( 2 )
758 - 782
DDYRPTWTLNQTEPVAGNYYPVNTR
Shared ( 2 )
931 NLS
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Serum Skeletal_muscle Stomach Testis
ConA Amide80 ConA RCA120 ConA AAL ConA AAL(+) AAL(+) AAL ConA RCA120 SSA RCA120 RCA120 AAL ConA Amide80 ConA ConA ConA RCA120
                         
               
                                           
       
     
 
 
     
 
                 
 
       
       
       
     
                                     
   
                                     
   
 
 
     
         
 
 
 
                                     
   
                                     
   
                                           
                                           
                                           
         
     
 
 
     
 
 
                       
             
           
 
 
               
                                           
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05