GlycoProtDB ID - GPDB0005160


Protein Name Aminopeptidase A
Protein Accession Number Q52JJ6
Gene Enpep
Length 945
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
MNFAEEEPSK KYCIKGKHVA IICGVVVAVG LIVGLSVGLT RSCEQDTTPA PSQPPPEAST ALPPQDQNVC PDSEDESGEW KNFRLPDFIN PVHYDLEVKA
LMEEDRYTGI VTISVNLSKP TRDLWLHIRE TKITKLPELR RPSGEQVPIR RCFEYKKQEY VVIQAAEDLA ATSGDSVYRL TMEFKGWLNG SLVGFYKTTY
MEDGQIRSIA ATDHEPTDAR KSFPCFDEPN KKSTYSISII HPKEYSALSN MPEEKSEMVD DNWKKTTFVK SVPMSTYLVC FAVHRFTAIE RKSRSGKPLK
VYVQPNQKET AEYAANITQA VFDYFEDYFA MEYALPKLDK IAIPDFGTGA MENWGLVTYR ETNLLYDPLL SASSNQQRVA SVVAHELVHQ WFGNTVTMDW
WDDLWLNEGF ASFFEFLGVN HAEKDWQMLS QVLLEDVFPV QEDDSLMSSH PVVVTVSTPA EITSVFDGIS YSKGASILRM LQDWITPEKF QKGCQIYLKK
FQFANAKTSD FWDSLQEASN LPVKEVMDTW TSQMGYPVVT VSGRQNITQK RFLLDSKADP SQPPSELGYT WNIPVRWADN DNSRITVYNR LDKGGITLNA
NLSGDAFLKI NPDHIGFYRV NYEGGTWDWI AEALSSNHTR FSAADRSSFI DDAFALARAQ LLNYKIALNL TMYLKSEEDF LPWERVISSV SYIISMFEDD
RELYPMIETY FQGQVKPVAD LLGWQDTGSH ITKLLRASIL GFACKMGDRE ALGNASQLFD SWLKGSASIP VNLRLLVYRY GMQNSGNEAA WNYTLEQYQK
TSLAQEKEKL LYGLASVKDV KLLARYLEML KDPNIIKTQD VFTVIRYISY NSYGKTMAWN WIQLNWDYLV SRFTINDRYL GRIVTIAEPF NTELQLWQMQ
SFFAKYPNAG AGAKPREQVL ETVKNNIEWL NVNRQSIREW FASLP

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
68 NVC
116 NLS
189 NGS 186 - 197
GWLNGSLVGFYK
Shared ( 3 )
316 NIT
546 NIT 525 - 550
EVMDTWTSQMGYPVVTVSGRQNITQK
Shared ( 5 )
525 - 550
EVMDTWTSQMGYPVVTVSGRQNITQK
Shared ( 5 )
601 NLS 591 - 609
LDKGGITLNANLSGDAFLK
Shared ( 4 )
594 - 609
GGITLNANLSGDAFLK
Shared ( 4 )
637 NHT 620 - 640
VNYEGGTWDWIAEALSSNHTR
Shared ( 3 )
669 NLT 666 - 675
IALNLTMYLK
Shared ( 3 )
666 - 675
IALNLTMYLK
Shared ( 3 )
754 NAS 750 - 764
EALGNASQLFDSWLK
Shared ( 3 )
746 - 764
MGDREALGNASQLFDSWLK
Shared ( 3 )
746 - 758
MGDREALGNASQL
Shared ( 3 )
746 - 764
MGDREALGNASQLFDSWLK
Shared ( 3 )
792 NYT 780 - 800
YGMQNSGNEAAWNYTLEQYQK
Shared ( 3 )
780 - 800
YGMQNSGNEAAWNYTLEQYQK
Shared ( 3 )
775 - 800
LLVYRYGMQNSGNEAAWNYTLEQYQK
Shared ( 3 )
781 - 800
GMQNSGNEAAWNYTLEQYQK
Shared ( 3 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Skeletal_muscle Stomach
RCA120 Amide80 Amide80 RCA120 AAL ConA RCA120 AAL(+) AAL(+) RCA120 SSA RCA120 RCA120 ConA RCA120 Amide80 RCA120 Amide80 RCA120
                                     
                                     
         
   
 
       
                                     
 
       
               
 
             
     
           
           
                       
           
   
     
   
 
     
   
   
 
 
     
   
 
 
       
     
   
   
       
     
         
   
 
   
       
               
                   
               
                   
 
         
           
 
 
 
   
   
     
       
       
 
 
   
       
             
                   
               
                   
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05