GlycoProtDB ID - GPDB0005379


Protein Name Ace protein (Fragment)
Protein Accession Number Q5XK22
Gene Ace
Length 1187
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
1001
1101
EVVMFQSTVA SWAHDTNITE ENARRQEEAA LVSQEFAEVW GKKAKELYES IWQNFTDSKL RRIIGSIRTL GPANLPLAQR QQYNSLLSNM SRIYSTGKVC
FPNKTATCWS LDPELTNILA SSRSYAKLLF AWEGWHDAVG IPLKPLYQDF TAISNEAYRQ DDFSDTGAFW RSWYESPSFE ESLEHIYHQL EPLYLNLHAY
VRRALHRRYG DKYVNLRGPI PAHLLGDMWA QSWENIYDMV VPFPDKPNLD VTSTMVQKGW NATHMFRVSE EFFTSLGLSP MPPEFWAESM LEKPTDGREV
VCHASAWDFY NRKDFRIKQC TRVTMEQLAT VHHEMGHVQY YLQYKDLHVS LRRGANPGFH EAIGDVLALS VSTPAHLHKI GLLDHVTNDI ESDINYLLKM
ALEKIAFLPF GYLVDQWRWG VFSGRTPPSR YNFDWWYLRT KYQGICPPVA RNETHFDAGA KFHIPNVTPY IRYFVSFVLQ FQFHQALCKE AGHQGPLHQC
DIYQSAQAGA KLKQVLQAGC SRPWQEVLKD LVGSDALDAK ALLEYFQPVS QWLEEQNQRN GEVLGWPENQ WRPPLPDNYP EGIDLETDEA KADRFVEEYD
RTAQVLLNEY AEANWQYNTN ITIEGSKILL EKSTEVSNHT LKYGTRAKTF DVSNFQNSSI KRIIKKLQNL DRAVLPPKEL EEYNQILLDM ETTYSLSNIC
YTNGTCMPLE PDLTNMMATS RKYEELLWAW KSWRDKVGRA ILPFFPKYVE FSNKIAKLNG YTDAGDSWRS LYESDNLEQD LEKLYQELQP LYLNLHAYVR
RSLHRHYGSE YINLDGPIPA HLLGNMWAQT WSNIYDLVAP FPSAPNIDAT EAMIKQGWTP RRIFKEADNF FTSLGLLPVP PEFWNKSMLE KPTDGREVVC
HPSAWDFYNG KDFRIKQCTS VNMEDLVIAH HEMGHIQYFM QYKDLPVTFR EGANPGFHEA IGDIMALSVS TPKHLYSLNL LSTEGSGYEY DINFLMKMAL
DKIAFIPFSY LIDQWRWRVF DGSITKENYN QEWWSLRLKY QGLCPPVPRS QGDFDPGSKF HVPANVPYVR YFVSFIIQFQ FHEALCRAAG HTGPLHKCDI
YQSKEAGKLL ADAMKLGYSK PWPEAMKLIT GQPNMSASAM MNYFKPLTEW LVTENRRHGE TLGWPEYNWA PNTGTTPTLP PAPGPSS

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
17 NIT
54 NFT 46 - 59
ELYESIWQNFTDSK
Shared ( 4 )
89 NMS 81 - 92
QQYNSLLSNMSR
Shared ( 4 )
81 - 92
QQYNSLLSNMSR
Shared ( 4 )
81 - 92
QQYNSLLSNMSR
Shared ( 4 )
103 NKT 99 - 123
VCFPNKTATCWSLDPELTNILASSR
Shared ( 4 )
261 NAT 259 - 267
GWNATHMFR
Shared ( 6 )
259 - 267
GWNATHMFR
Shared ( 6 )
452 NET 442 - 461
YQGICPPVARNETHFDAGAK
Shared ( 6 )
452 - 461
NETHFDAGAK
Shared ( 6 )
466 NVT
620 NIT
638 NHT 633 - 642
STEVSNHTLK
Shared ( 7 )
633 - 646
STEVSNHTLKYGTR
Shared ( 7 )
657 NSS 647 - 661
AKTFDVSNFQNSSIK
Shared ( 7 )
649 - 662
TFDVSNFQNSSIKR
Shared ( 7 )
649 - 661
TFDVSNFQNSSIK
Shared ( 7 )
647 - 662
AKTFDVSNFQNSSIKR
Shared ( 7 )
698 NIC
703 NGT
885 NKS 866 - 886
EADNFFTSLGLLPVPPEFWNK
Shared ( 7 )
1134 NMS 1128 - 1156
LITGQPNMSASAMMNYFKPLTEWLVTENR
Shared ( 8 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Serum Skeletal_muscle Stomach Testis
ConA RCA120 Amide80 Amide80 ConA RCA120 AAL ConA RCA120 AAL(+) AAL(+) ConA RCA120 RCA120 RCA120 AAL ConA RCA120 Amide80 Amide80 ConA RCA120 RCA120 AAL ConA RCA120
                                                   
           
   
       
 
           
 
 
 
 
 
 
   
   
       
 
       
             
     
           
                             
         
     
     
           
           
 
           
             
         
     
   
 
     
   
                                       
         
   
       
         
                 
                                                   
                                                   
         
 
     
           
         
                                       
             
                                   
       
   
     
         
     
   
       
             
                                   
             
                                                   
                                                   
     
         
                 
           
             
                                   
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05