GlycoProtDB ID - GPDB0005473


Protein Name Biliary glycoprotein
Protein Accession Number Q61351
Gene Ceacam1
Length 458
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
MELASAHLHK GQVPWVGLLL TASLLASWSP PTTAEVTIEA VPPQVAEDNN VLLLVHNLPL ALGAFAWYKG NPVSTNAEIV HFVTGTNKTT TGPAHSGRET
VYSNGSLLIQ RVTVKDTGVY TIEMTDENFR RTEATVQFHV HQLLLKPNIT SNNSNPVEGD DSVSLTCDSY TDPDNITYLW SRNGESLSEG DRLKLSEGNR
T
LTLLNVTRN DTGPYVCETR NPVSVNRSDP FSLNIIYGPD TPIISPSDIY LHPGSNLNLS CHAASNPPAQ YFWLINEKPH ASSQELFIPN ITTNNSGTYT
CLVNNSVTGL SRTTVKNITV LEPVTQPSLQ VTNTTVKELD SVTLTCLSND IGANIQWLFN SQSLQLTERM TLSQNNSILR IDPIKREDAG EYQCEISNPV
SVKRSNSIKL DIIFDPTQGG LSDGAIAGIV IGVVAGVALI AGLAYFLYSR KSGGSGSF

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
87 NKT
104 NGS
148 NIT
152 NNS
175 NIT
199 NRT 195 - 209
LSEGNRTLTLLNVTR
Shared ( 8 )
195 - 220
LSEGNRTLTLLNVTRNDTGPYVCETR
Shared ( 8 )
206 NVT 195 - 209
LSEGNRTLTLLNVTR
Shared ( 8 )
195 - 220
LSEGNRTLTLLNVTRNDTGPYVCETR
Shared ( 8 )
201 - 220
TLTLLNVTRNDTGPYVCETR
Shared ( 8 )
201 - 209
TLTLLNVTR
Shared ( 8 )
201 - 227
TLTLLNVTRNDTGPYVCETRNPVSVNR
Shared ( 8 )
210 NDT 195 - 220
LSEGNRTLTLLNVTRNDTGPYVCETR
Shared ( 8 )
201 - 220
TLTLLNVTRNDTGPYVCETR
Shared ( 8 )
210 - 220
NDTGPYVCETR
Shared ( 8 )
210 - 227
NDTGPYVCETRNPVSVNR
Shared ( 8 )
201 - 227
TLTLLNVTRNDTGPYVCETRNPVSVNR
Shared ( 8 )
226 NRS 210 - 227
NDTGPYVCETRNPVSVNR
Shared ( 8 )
201 - 227
TLTLLNVTRNDTGPYVCETRNPVSVNR
Shared ( 8 )
258 NLS
290 NIT
294 NNS
304 NNS
317 NIT
333 NTT
375 NNS 370 - 385
MTLSQNNSILRIDPIK
Shared ( 14 )
370 - 385
MTLSQNNSILRIDPIK
Shared ( 14 )
370 - 380
MTLSQNNSILR
Shared ( 14 )
370 - 380
MTLSQNNSILR
Shared ( 14 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Skeletal_muscle Stomach
RCA120 Amide80 ConA RCA120 Amide80 RCA120 AAL ConA RCA120 AAL(+) AAL(+) AAL ConA RCA120 SSA WGA RCA120 RCA120 RCA120 ConA RCA120 Amide80 RCA120
                                             
                                             
                                             
                                             
                                             
                               
         
                               
         
                               
         
                               
         
   
     
 
 
 
     
                         
 
         
                                 
         
                               
         
   
     
 
 
 
     
                               
         
                               
         
                                 
         
                               
         
                                 
         
                                             
                                             
                                             
                                             
                                             
                                             
 
   
                                   
 
                                         
     
 
 
     
       
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05