GlycoProtDB ID - GPDB0005489


Protein Name CD166 antigen
Protein Accession Number Q61490
Gene Alcam
Length 583
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
MASKVSPSCR LVFCLLISAA VLRPGLGWYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI
ANAKISDEKR FVCMLVTEDN VFEAPTLVKV FKQPSKPEIV NKAPFLETDQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV EGEVAILFKK EIDPGTQLYT
VTSSLEYKTT RSDIQMPFTC SVTYYGPSGQ KTIYSEQEIF DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQPE GIRSSNTYTL
TDVRRNATGD YKCSLIDKRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE
GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIHWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV
S
AISIPEHDE ADDISDENRE KVNDQAKLIV GIVVGLLLAA LVAGVVYWLY MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
95 NYT 90 - 104
LSLSENYTLSIANAK
Shared ( 5 )
167 NIT 161 - 172
DSYPDGNITWYR
Shared ( 5 )
265 NIT
306 NAT 294 - 312
SSNTYTLTDVRRNATGDYK
Shared ( 7 )
361 NAT 345 - 368
QIGDTLPVSCTISASRNATVVWMK
Shared ( 5 )
361 - 368
NATVVWMK
Shared ( 5 )
361 - 368
NATVVWMK
Shared ( 5 )
361 - 372
NATVVWMKDNIR
Shared ( 5 )
457 NQT
480 NVT 473 - 493
IIISPEENVTLTCTAENQLER
Shared ( 11 )
499 NVS 494 - 521
TVNSLNVSAISIPEHDEADDISDENREK
Shared ( 4 )
Brain Colon Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Stomach
ConA RCA120 Amide80 RCA120 RCA120 AAL(+) AAL(+) AAL RCA120 RCA120 RCA120 ConA RCA120 Amide80 RCA120
   
     
 
   
 
     
 
   
                             
     
         
       
   
                       
               
   
 
           
           
               
     
   
                             
   
   
     
 
   
           
   
 
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05