GlycoProtDB ID - GPDB0005680


Protein Name Autophagy-related protein 9A
Protein Accession Number Q68FE2
Gene Atg9a
Length 551
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
MAQFDTEYQR LEASYSDSPP GEEDLLVHVA EGSKSPWHHI ENLDLFFSRV YNLHQKNGFT CMLIGEMFEL MQFLFVVAFT TFLVSCVDYD ILFANKMVNH
S
LHPTEPVKV TLPDAFLPAQ VCSARIQENG SLITILVIAG VFWIHRLIKF IYNICCYWEI HSFYLHALRI PMSALPYCTW QEVQARIVQT QKEHQICIHK
RELTELDIYH RILRFQNYMV ALVNKSLLPL RFRLPGLGEV VFFTRGLKYN FELILFWGPG SLFLNEWSLK AEYKRGGQRL ELAQRLSNRI LWIGIANFLL
CPLILIWQIL YAFFSYAEVL KREPGALGAR CWSLYGRCYL RHFNELEHEL QSRLNRGYKP ASKYMNCFLS PLLTLLAKNG AFFAGSILAV LIALTIYDED
VLAVEHVLTT VTLLGVTVTV CRSFIPDQHM VFCPEQLLRV ILAHIHYMPD HWQGVHLGGV AESHRHTPHS HLLPPPSGPG DHRLLPQLYG RGRGCGRHLL
LCSDGRSPAW PSSVAVWRAD RGLSVPASRG REDRVVAHAL CHHQSRLAAP S

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
99 NHS 97 - 109
MVNHSLHPTEPVK
Shared ( 18 )
97 - 109
MVNHSLHPTEPVK
Shared ( 18 )
129 NGS
153 NIC
224 NKS
Heart Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Skeletal_muscle Stomach
ConA RCA120 AAL(+) ConA RCA120 WGA RCA120 RCA120 ConA ConA RCA120
   
   
 
     
                     
                     
                     
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05