GlycoProtDB ID - GPDB0005792


Protein Name Anoctamin-6
Protein Accession Number Q6P9J9
Gene Ano6
Length 911
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
MQMMTRKVLL NMELEEDDDE DGDIVLENFD QTIVCPTFGS LENQQDFRTP EFEEFNGKPD SLFFTDGQRR IDFILVYEDE SKKENNKKGT NEKQKRKRQA
YESNLICHGL QLEATRSVSD DKLVFVKVHA PWEVLCTYAE IMHIKLPLKP NDLKTRSPFG NLNWFTKVLR VNESVIKPEQ EFFTAPFEKS RMNDFYILDR
DSFFNPATRS RIVYFILSRV KYQVMNNVNK FGINRLVSSG IYKAAFPLHD CRFNYESEDI SCPSERYLLY REWAHPRSIY KKQPLDLIRK YYGEKIGIYF
AWLGYYTQML LLAAVVGVAC FLYGYLDQDN CTWSKEVCDP DIGGQILMCP QCDRLCPFWR LNITCESSKK LCIFDSFGTL IFAVFMGVWV TLFLEFWKRR
QAELEYEWDT VELQQEEQAR PEYEAQCNHV VINEITQEEE RIPFTTCGKC IRVTLCASAV FFWILLIIAS VIGIIVYRLS VFIVFSTTLP KNPNGTDPIQ
KYLTPQMATS ITASIISFII IMILNTIYEK VAIMITNFEL PRTQTDYENS LTMKMFLFQF VNYYSSCFYI AFFKGKFVGY PGDPVYLLGK YRSEECDPGG
CLLELTTQLT IIMGGKAIWN NIQEVLLPWV MNLIGRYKRV SGSEKITPRW EQDYHLQPMG KLGLFYEYLE MIIQFGFVTL FVASFPLAPL LALVNNILEI
RVDAWKLTTQ FRRMVPEKAQ DIGAWQPIMQ GIAILAVVTN AMIIAFTSDM IPRLVYYWSF SIPPYGDHTY YTMDGYINNT LSVFNITDFK NTDKENPYIG
LGNYTLCRYR DFRNPPGHPQ EYKHNIYYWH VIAAKLAFII VMEHIIYSVK FFISYAIPDV SKITKSKIKR EKYLTQKLLH ESHLKDLTKN MGIIAERIGG
TVDNSVRPKL E

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
172 NES
330 NCT
362 NIT 361 - 369
LNITCESSK
Unique
361 - 370
LNITCESSKK
Unique
494 NGT 492 - 501
NPNGTDPIQK
Shared ( 2 )
778 NNT
785 NIT
803 NYT 791 - 808
NTDKENPYIGLGNYTLCR
Shared ( 2 )
795 - 808
ENPYIGLGNYTLCR
Shared ( 2 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Skeletal_muscle Stomach Testis
ConA Amide80 RCA120 Amide80 ConA RCA120 ConA RCA120 AAL(+) AAL(+) RCA120 RCA120 ConA RCA120 RCA120 Amide80 RCA120 ConA
                                   
                                   
     
   
       
                       
         
         
   
 
           
                                   
                                   
           
         
       
 
 
   
   
 
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05