GlycoProtDB ID - GPDB0005943


Protein Name Acid ceramidase
Protein Accession Number Q78P93
Gene Asah1
Length 394
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
MRGQSLLTWV LAAAVTCAQA QDVPPWTEDC RKSTYPPSGP TYRGPVPWHT INLDLPPYKR WHELLAQKAP ALRILVNSIT SLVNTFVPSG KLMKMVDQKL
PGMIGSLPDP FGEEMRGIAD VTGIPLGEII SFNIFYELFT MCTSIITEDE KGHLLHGRNM DFGIFLGWNI NNNTWVVTEE LKPLTVNLDF QRNNKTVFKA
TSFVGYVGML TGFKPGLFSL SLNERFSING GYLGILEWMF GRKDAQWVGF ITRSVLENTT SYEEAKNTLT KTKIMAPVYF ILGGKKSGEG CVITRERKES
LDVYELDPKH GRWYVVQTNY DRWKNTLFID DRRTPAKKCL NHTTQKNLSF ATIYDVLSTK PVLNKLTVFT TLMDVTKGQF ESHLRDCPDP CIGW

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
172 NNT
194 NKT
258 NTT 254 - 271
SVLENTTSYEEAKNTLTK
Shared ( 5 )
254 - 266
SVLENTTSYEEAK
Shared ( 5 )
341 NHT
347 NLS 347 - 365
NLSFATIYDVLSTKPVLNK
Shared ( 5 )
Brain Colon Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Skeletal_muscle Testis
ConA ConA ConA AAL(+) AAL(+) AAL ConA RCA120 RCA120 RCA120 ConA ConA ConA
                         
                         
 
     
   
 
 
     
 
                         
 
   
             
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05