GlycoProtDB ID - GPDB0005966


Protein Name Biglycan
Protein Accession Number Q7TMW3
Gene Bgn
Length 369
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
MCPLWLLTLL LALSQALPFE QKGFWDFTLD DGLLMMNDEE ASGSDTTSGV PDLDSVTPTF SAMCPFGCHC HLRVVQCSDL GLKTVPKEIS PDTTLLDLQN
NDVSELRKDD FKGLQHLYAL VLVNNKISKI HEKAFSPLRK LQKLYISKNH LVEIPPNLPS SLVELRIHDN RIRKVPKGVF SGLRNMNCIE MGGNPLENSG
FEPGAFDGLK LNYLRISEAK LTGIPKDLPE TLNELHLDHN KIQAIELEDL LRYSKLYRLG LGHNQIRMIE NGSLSFLPTL RELHLDNNKL SRVPAGLPDL
KLLQVVYPHS NNITKVGIND FCPMGFGVKR AYYNGISLFN NPVPYWEVQP ATFRCVTDRL AIQFGNYKK

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
271 NGS 268 - 289
MIENGSLSFLPTLRELHLDNNK
Shared ( 5 )
268 - 281
MIENGSLSFLPTLR
Shared ( 5 )
268 - 281
MIENGSLSFLPTLR
Shared ( 5 )
312 NIT
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Skeletal_muscle Stomach Testis
RCA120 Amide80 RCA120 Amide80 ConA RCA120 AAL ConA RCA120 AAL(+) AAL(+) AAL ConA RCA120 RCA120 RCA120 AAL ConA RCA120 ConA RCA120 Amide80 RCA120 AAL ConA RCA120
 
                                     
       
         
       
 
   
                                                   
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05