GlycoProtDB ID - GPDB0006025


Protein Name C4b-binding protein alpha-chain
Protein Accession Number Q80SX2
Gene C4bp
Length 426
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
MTFTAALWVA VFGKCGPPPA IPNALPASDV NRTDFESHTT LKYECLPGYG RGISRMTVYC KPSGEWEISV SCAKKHCRNP GYLDNGYVNG ETITFGSQIE
FSCQEGFILV GSSTSSCEVR GKGVAWSNPF PECVIVKCGP PPDISNGKHS GTEDFYPYNH GISYTCDPGF RLVGSPFIGC TVVNKTVPVW SSSPPTCEKI
ICSQPNILHG VIVSGYKATY THRDSVRLAC LNGTVLRGRH VIECQGNGNW SSLPTCEFDC DLPPAIVNGY YTSMVYSKIT LVTYECDKGY RLVGKAIISC
SFSKWKGTAP QCKALCQKPE VGNGTLSDEK DQYVESENVT IQCDSGFAML GSQSISCSES GTWYPEVPRC EQEASEDLKP ALTGNKTMQY VPNSHDVKMA
LEIYKLTLEV ELLQLQIQKE KHTEAH

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
31 NRT 15 - 42
CGPPPAIPNALPASDVNRTDFESHTTLK
Shared ( 4 )
184 NKT 172 - 185
LVGSPFIGCTVVNK
Shared ( 5 )
172 - 199
LVGSPFIGCTVVNKTVPVWSSSPPTCEK
Shared ( 5 )
232 NGT 228 - 237
LACLNGTVLR
Shared ( 4 )
249 NWS
323 NGT 314 - 330
ALCQKPEVGNGTLSDEK
Shared ( 4 )
319 - 330
PEVGNGTLSDEK
Shared ( 4 )
338 NVT
385 NKT 370 - 386
CEQEASEDLKPALTGNK
Shared ( 3 )
370 - 398
CEQEASEDLKPALTGNKTMQYVPNSHDVK
Shared ( 3 )
370 - 398
CEQEASEDLKPALTGNKTMQYVPNSHDVK
Shared ( 3 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Serum Skeletal_muscle Stomach Testis
RCA120 RCA120 Amide80 ConA RCA120 RCA120 AAL(+) AAL(+) AAL ConA RCA120 SSA WGA RCA120 RCA120 RCA120 Amide80 RCA120 RCA120 AAL ConA RCA120
           
                           
 
   
   
                               
         
     
 
 
   
                                           
 
 
 
         
   
     
             
                           
                                           
           
   
         
         
                               
         
                               
         
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05