GlycoProtDB ID - GPDB0006096


Protein Name Abca3 protein (Fragment)
Protein Accession Number Q80XT2
Gene Abca3
Length 1538
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
1001
1101
1201
1301
1401
1501
QTGNIFLKET EGWHTTSLFP LFPSPGPREP SSPDGGEPGY IREGFLAMQH AVDKAIMRYH ANTSAQQLFQ KLMVITKRFP FPPYISDPFL IAIQYQLPLL
LMLSFTYTSL TIIRAVVQEK EKKLKEYMRM MGLNSWLHWS AWFLMFFLFF LIVVSFMTLL FCVKVKKDIA VLSNSDPSLV LAFLLCFAIS SISFSFMVST
FFSKANIAAA VGGFLYFFTY TPYFFVAPRY NWMTLSQKLL SCLLSNVAMA MGAQLIGKFE AKGTGIQWRD LLNPVNVDDD FCFGQVLGML LLDSALYGLV
TWYVEAVFPG QFGVPQPWHF FLMPSYWCGN PRTVVGKEEE GSDPEKALRN EYFEAEPEDL VAGIKIKHLS KVFQVGNKDK MGIRDLTLNL YEGQITVLLG
HNGAGKTTTM SLLTGLFPPT SGHAYIHGYE ISQDMAQIRK SLGLCPQHDV LFDNLTVAEH LYFYAQLKGL SLQKCPEEVK QMLHILSLED KRDLRSKFLS
GGMKRKLSIG IALIAGSKVL MLDEPTSGMD AVSRRAIWDL LQQQKSDRTV LLTTHFMDEA DLLGDRIAIL AKGELQCCGS SLFLKQKYGA GYHMTLVKEP
HCNPEGISQL VHHHVPNAML ESHAGAELSF ILPKESTHRF ESLFAKLEKK QKELGIASFG ASVTTMEEVF LRVGKLVDTS MDIQAIQLPA LQYQHERRAS
DWALDSNLCG VMDPTNGIGA LIEEEEVLVK LNTGLALHCQ QFWAMFLKKA AYSWREWKMV AAQVLVPLTC LTLALLAIHY TSEIFDDPLL KLSLNEYGRT
VVPFSVPSTS RLAQQLSENL RDMLQAERQE PREVLGDLEE FLVFRASVEG GGFNERCLVA TSFKDRGELT VVTALFNNQA YHSPATALAI VDNLLFKLLC
GPRASIEISN YPQPRNTLQV AKDHFNEGRK GFDIALNLLI AMAFLASTFS ILAVSERAVQ AKHVQFVSGV HVATFWLSAL LWDLISFLVP SLLLLVVFQA
FNVHAFTRDG HMADLLLLLM LYGWAIIPLM YLMSFFFSAA STAYTRLTIF NILSGIATFI MVTIMRIPAV KLEELSRTLD HVFLVLPNHC LGMAVSNFYE
NYETRRYCTS SELAAHYCKK YNIQYQESFY AWSTPGVGKF VTSMAASGGI YLTLLFLIET NLLWRLRTFI CAFRRRWTLA ELQNRTSVLP EDQDVAEERS
RILVPSLDSM LDTPLIINEL SKVYDQRAPL LAVDRISLAV QKGECFGLLG FNGAGKTTTF KMLTGEETIT SGDAFVGGYS ISSDIGKVRQ RMGYCPQFDA
LLDHMTGREM LVMYARLRGI PERLINACVE NTLRGLLLEP HANKLVKTYS GGNKRKLSTG IALIGEPAVI FLDEPSTGMD PVARRLLWDT VARARESGKA
IVITSHSMEE CEALCTRLAI MVQGQFKCLG SPQHLKSKFG SGYSLQAKVR SEGKQDALEE FKAFVDLTFP GSILEDEHQD MVHYHLPGCD LSWAKVFGIL
EKAKEKYGVD DYSVSQISLE QVFLSFAHLQ PPTTEDGR

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
62 NTS 59 - 71
YHANTSAQQLFQK
Shared ( 4 )
454 NLT
707 NLC
1088 NHC
1184 NRT
1326 NAC
Brain Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung
ConA AAL AAL(+) AAL(+) RCA120 RCA120 ConA
             
             
             
             
             
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05