GlycoProtDB ID - GPDB0006346


Protein Name Alpha-mannosidase 2x
Protein Accession Number Q8BRK9
Gene Man2a2
Length 1152
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
1001
1101
MKLKKQVTVC GAAIFCVAVF SLYLMLDRVQ HDPARHQNGG NFPRSQISVL QNRIEQLEQL LEENHDIISR IKDSVLELTA NAEGPPALLP YHTANGSWAV
LPEPRPSFFS VSPQDCQFAL GGRGQKPELQ MLTVSEDLPF DNVEGGVWRQ GFDISYSPND WDTEDLQVFV VPHSHNDPGW IKTFDKYYTE QTQHILNSMV
SKLQEDPRRR FLWAEVSFFA KWWDNISAQK RAAVRRLVGN GQLEIATGGW VMPDEANSHY FALVDQLIEG HQWLERNLGA TPRSGWAVDP FGHSSTMPYL
LRRANLTSML IQRVHYAIKK HFAATHSLEF MWRQMWDSDS STDIFCHMMP FYSYDVPHTC GPDPKICCQF DFKRLPGGRI NCPWKVPPRA ITEANVADRA
ALLLDQYRKK SRLFRSNVLL VPLGDDFRYD KPQEWDAQFF NYQRLFDFLN SKPEFHVQAQ FGTLSEYFDA LYKRTGVEPG ARPPGFPVLS GDFFSYADRE
DHYWTGYYTS RPFYKSLDRV LEAHLRGAEI LYSLALAHAR RSGLAGQYPL SDFALLTEAR RTLGLFQHHD AITGTAKEAV VVDYGVRLLR SLVSLKQVII
NAAHYLVLGD QETYSFDPGT PFLQMDDSRV SHDALPERTV IRLDSSPRFV VVFNPLEQER LSVVSLLVNS PRVRVLSEEG QPLSVQISVH WSSATDMVPD
VYQVSVPVRL PGLGLGVLQL QPDLDGPYTL QSSVRVYLNG VKLSVSRQSA FPVRVVDSGA SDFAISNRYM QVWFSGLTGL LKSIRRVDEE QEQQMELEFL
VYGTRTSKDK SGAYLFLPDS EAKPYVPKKP PVLRVTEGPF FSEVAVYYEH FHQVIRLYNL PGVEGLSLDM SFQVDIRDYV NKELALRIHT DIDSQGTFFT
DLNGFQIQPR QYLKKLPLQA NFYPMPVMAY IQDSQRRLTL HTAQALGVSS LGNGQLEVIL DRRLMQDDNR GLGQGLKDNK ITCNRFRLLL ERRTTMSPEV
HQEQERSTSY PSLLSHLTSM YLSTPPLVLP VAKRQGTSPA LRSFHPLASP LPCDFHLLNL RMLPAEDTLP ATDSALILHR KGFDCGLEAK NLGFNCTTSQ
GKLALGSLFH GLDVTFLQPT SLTLLYPLAS PSNSTDISLE PMEISTFRLR LG

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
95 NGS
225 NIS
305 NLT 304 - 313
ANLTSMLIQR
Shared ( 5 )
1095 NCT 1091 - 1102
NLGFNCTTSQGK
Shared ( 12 )
1133 NST
Kidney(Fut9 KO) Kidney(Fut9 WT) Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Testis
AAL(+) AAL(+) RCA120 RCA120 RCA120
         
         
   
     
         
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05