GlycoProtDB ID - GPDB0006758


Protein Name Adhesion G-protein coupled receptor G1
Protein Accession Number Q8K209
Gene Adgrg1
Length 687
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
MAVQVLRQMV YFLLSLFSLV QGAHSGSPRE DFRFCGQRNQ TQQSTLHYDQ SSEPHIFVWN TEETLTIRAP FLAAPDIPRF FPEPRGLYHF CLYWSRHTGR
LHLRYGKHDY LLSSQASRLL CFQKQEQSLK QGAPLIATSV SSWQIPQNTS LPGAPSFIFS FHNAPHKVSH NASVDMCDLK KELQQLSRYL QHPQKAAKRP
TAAFISQQLQ SLESKLTSVS FLGDTLSFEE DRVNATVWKL PPTAGLEDLH IHSQKEEEQS EVQAYSLLLP RAVFQQTRGR RRDDAKRLLV VDFSSQALFQ
DKNSSQVLGE KVLGIVVQNT KVTNLSDPVV LTFQHQPQPK NVTLQCVFWV EDPASSSTGS WSSAGCETVS RDTQTSCLCN HLTYFAVLMV SSTEVEATHK
HYLTLLSYVG CVISALACVF TIAAYLCSRR KSRDYTIKVH MNLLSAVFLL DVSFLLSEPV ALTGSEAACR TSAMFLHFSL LACLSWMGLE GYNLYRLVVE
VFGTYVPGYL LKLSIVGWGF PVFLVTLVAL VDVNNYGPII LAVRRTPERV TYPSMCWIRD SLVSYVTNLG LFSLVFLFNL AMLATMVVQI LRLRPHSQNW
PHVLTLLGLS LVLGLPWALV FFSFASGTFQ LVILYLFSII TSFQGFLIFL WYWSMRFQAQ GGPSPLKNNS DSAKLPISSG STSSSRI

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
39 NQT
148 NTS
171 NAS 168 - 180
VSHNASVDMCDLK
Shared ( 4 )
168 - 181
VSHNASVDMCDLKK
Shared ( 4 )
168 - 180
VSHNASVDMCDLK
Shared ( 4 )
234 NAT
303 NSS 303 - 311
NSSQVLGEK
Unique
324 NLS 322 - 340
VTNLSDPVVLTFQHQPQPK
Unique
341 NVT
668 NNS
Brain Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Stomach
ConA RCA120 Amide80 ConA AAL(+) AAL(+) RCA120 RCA120 ConA ConA
                   
                   
     
     
       
     
       
     
                   
       
       
 
 
                   
                   
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05