GlycoProtDB ID - GPDB0006759


Protein Name Ace protein (Fragment)
Protein Accession Number Q8K233
Gene Ace
Length 1015
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
901
1001
WYESPSFEES LEHIYHQLEP LYLNLHAYVR RALHRRYGDK YVNLRGPIPA HLLGDMWAQS WENIYDMVVP FPDKPNLDVT STMVQKGWNA THMFRVSEEF
FTSLGLSPMP PEFWAESMLE KPTDGREVVC HASAWDFYNR KDFRIKQCTR VTMEQLATVH HEMGHVQYYL QYKDLHVSLR RGANPGFHEA IGDVLALSVS
TPAHLHKIGL LDHVTNDIES DINYLLKMAL EKIAFLPFGY LVDQWRWGVF SGRTPPSRYN FDWWYLRTKY QGICPPVARN ETHFDAGAKF HIPNVTPYIR
YFVSFVLQFQ FHQALCKEAG HQGPLHQCDI YQSAQAGAKL KQVLQAGCSR PWQEVLKDLV GSDALDAKAL LEYFQPVSQW LEEQNQRNGE VLGWPENQWR
PPLPDNYPEG IDLETDEAKA DRFVEEYDRT AQVLLNEYAE ANWQYNTNIT IEGSKILLEK STEVSNHTLK YGTRAKTFDV SNFQNSSIKR IIKKLQNLDR
AVLPPKELEE YNQILLDMET TYSLSNICYT NGTCMPLEPD LTNMMATSRK YEELLWAWKS WRDKVGRAIL PFFPKYVEFS NKIAKLNGYT DAGDSWRSLY
ESDNLEQDLE KLYQELQPLY LNLHAYVRRS LHRHYGSEYI NLDGPIPAHL LGNMWAQTWS NIYDLVAPFP SAPNIDATEA MIKQGWTPRR IFKEADNFFT
SLGLLPVPPE FWNKSMLEKP TDGREVVCHP SAWDFYNGKD FRIKQCTSVN MEDLVIAHHE MGHIQYFMQY KDLPVTFREG ANPGFHEAIG DIMALSVSTP
KHLYSLNLLS TEGSGYEYDI NFLMKMALDK IAFIPFSYLI DQWRWRVFDG SITKENYNQE WWSLRLKYQG LCPPVPRSQG DFDPGSKFHV PANVPYVRYF
VSFIIQFQFH EALCRAAGHT GPLHKCDIYQ SKEAGKLLAD AMKLGYSKPW PEAMKLITGQ PNMSASAMMN YFKPLTEWLV TENRRHGETL GWPEYNWAPN
TGTTPTLPPA PGPSS

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
89 NAT 87 - 95
GWNATHMFR
Shared ( 6 )
87 - 95
GWNATHMFR
Shared ( 6 )
280 NET 270 - 289
YQGICPPVARNETHFDAGAK
Shared ( 6 )
280 - 289
NETHFDAGAK
Shared ( 6 )
294 NVT
448 NIT
466 NHT 461 - 470
STEVSNHTLK
Shared ( 7 )
461 - 474
STEVSNHTLKYGTR
Shared ( 7 )
485 NSS 475 - 489
AKTFDVSNFQNSSIK
Shared ( 7 )
477 - 490
TFDVSNFQNSSIKR
Shared ( 7 )
477 - 489
TFDVSNFQNSSIK
Shared ( 7 )
475 - 490
AKTFDVSNFQNSSIKR
Shared ( 7 )
526 NIC
531 NGT
713 NKS 694 - 714
EADNFFTSLGLLPVPPEFWNK
Shared ( 7 )
962 NMS 956 - 984
LITGQPNMSASAMMNYFKPLTEWLVTENR
Shared ( 8 )
Brain Colon Heart Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung Serum Skeletal_muscle Stomach Testis
ConA RCA120 Amide80 Amide80 ConA RCA120 AAL ConA RCA120 AAL(+) AAL(+) ConA RCA120 RCA120 RCA120 AAL ConA RCA120 Amide80 Amide80 ConA RCA120 RCA120 AAL ConA RCA120
           
 
           
             
         
     
   
 
     
   
                                       
         
   
       
         
                 
                                                   
                                                   
         
 
     
           
         
                                       
             
                                   
       
   
     
         
     
   
       
             
                                   
             
                                                   
                                                   
     
         
                 
           
             
                                   
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05