GlycoProtDB ID - GPDB0006772


Protein Name Beta-mannosidase
Protein Accession Number Q8K2I4
Gene Manba
Length 879
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
501
601
701
801
MHLHLLLILA LFRAGCVVAG PSYSLSGSWR VSNGNGSLEL PATVPGYVHS ALHQHGLIQD PYYRFNDLNY RWISLDNWTY STEFKIPFNL SEWQKVKLIF
DGVDTVAEIL FNNVTIGKTD NMFTGYSFDI TNVVKDVNSL KLQFRSAVQY AECQSKAHTS YRVPPECPPV EQKGECHVNF IRKAQCSFSW DWGPSFPSQG
IWKDVRIEAY NIAHLDYLTF LPVYDNASQA WNIEIKASFD VASSKSVGGQ VTVAIPQLKT QQTNDIELQQ EQRIVKLLVK IRKDVAVETW WPRGHGNQTG
YNMTILFALD GGLKIEKAAK VYFRTVQLIE EGIKGSPGLS FYFKINGLPI FLKGSNWIPA DSFQDKVTSD RLQLLFQSVV DANMNTLRVW GGGIYEQDEF
YALCDELGIM VWQDFMFASA LYPTEPGFLA SVRKEVTYQV RRLKSHPSII IWSGNNENEV ALSVNWFHVN PRDMKTYIDD YVTLYVKNIR KIVLSEDKSR
PFIASSPTNG MKTMEEGWIS YDPYSIQYGD IHFYNYADDC WNWKIFPKAR LVSEYGYQSW PSFSTLEKVS SQEDWAYNSR FSLHRQHHED GNHQMLHQVK
MHFKLPQGTD PLRTFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVDGKGH TMGALYWQLN DIWQAPSWAS LEYGGKWKML HYFARRFFAP LLPVGFEDEG
VFYVYGVSDL HKDHHTQLTV RLHHWSSPKP LCSLVNSSIV VKAGEAVVLF QMPVSELLKR CRGCTRETCV VSFYFSTDKE LFSPTNYHFL SSLKDAKGLL
EANITVNISQ KGNVFVFDLE TSAVAPFVWL DVGSIPGRFS DNGFLMIRKK LSVLFYPWKP TSKSELQQAF SVTSLTDTY

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
35 NGS
77 NWT
89 NLS 86 - 95
IPFNLSEWQK
Shared ( 3 )
113 NVT
226 NAS
297 NQT 294 - 314
GHGNQTGYNMTILFALDGGLK
Shared ( 2 )
294 - 314
GHGNQTGYNMTILFALDGGLK
Shared ( 2 )
302 NMT 294 - 314
GHGNQTGYNMTILFALDGGLK
Shared ( 2 )
294 - 314
GHGNQTGYNMTILFALDGGLK
Shared ( 2 )
294 - 314
GHGNQTGYNMTILFALDGGLK
Shared ( 2 )
736 NSS
803 NIT
807 NIS
Heart Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Skeletal_muscle Stomach
ConA AAL(+) AAL(+) RCA120 RCA120 RCA120 ConA ConA
               
               
   
   
               
               
       
     
   
       
     
       
     
   
               
               
               
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05