GlycoProtDB ID - GPDB0006956


Protein Name C-type lectin domain family 14 member A
Protein Accession Number Q8VCP9
Gene Clec14a
Length 459
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
MRPALALCLL CPAFWPRPGN GEHPTADRAA CSASGACYSL HHATFKRRAA EEACSLRGGT LSTVHSGSEF QAVLLLLRAG PGPGGGSKDL LFWVALERSI
SQCTQEKEPL RGFSWLHPDS EDSEDSPLPW VEEPQRSCTV RKCAALQATR GVEPAGWKEM RCHLRTDGYL CKYQFEVLCP APRPGAASNL SFQAPFRLSS
SALDFSPPGT EVSAMCPGDL SVSSTCIQEE TSAHWDGLFP GTVLCPCSGR YLLAGKCVEL PDCLDHLGDF TCECAVGFEL GKDGRSCETK VEEQLTLEGT
KLPTRNVTAT PAGAVTNRTW PGQVYDKPGE MPQVTEILQW GTQSTLPTIQ KTPQTKPKVT GTPSGSVVLN YTSSPPVSLT FDTSSTVVFI LVSIAVIVLV
VLTITVLGLF KLCFHKSRSS RTGKGALDSP GVECDAEATS LHHSSTQCTD IGVKSGTVA

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
189 NLS 173 - 197
YQFEVLCPAPRPGAASNLSFQAPFR
Unique
306 NVT
317 NRT
370 NYT
Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-)
AAL(+) AAL(+) AAL RCA120 RCA120 RCA120
           
           
           
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05