GlycoProtDB ID - GPDB0007103


Protein Name CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4
Protein Accession Number Q91Y74
Gene St3gal4
Length 333
Status
Reviewed
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
MTSKSHWKLL ALALVLVVVM VWYSISREDR YIEFFYFPIS EKKEPCFQGE AERQASKIFG NRSREQPIFL QLKDYFWVKT PSTYELPFGT KGSEDLLLRV
LAITSYSIPE SIKSLECRRC VVVGNGHRLR NSSLGGVINK YDVVIRLNNA PVAGYEGDVG SKTTIRLFYP ESAHFDPKIE NNPDTLLVLV AFKAMDFHWI
ETILSDKKRV RKGFWKQPPL IWDVNPKQVR ILNPFFMEIA ADKLLSLPIQ QPRKIKQKPT TGLLAITLAL HLCDLVHIAG FGYPDASNKK QTIHYYEQIT
LKSMAGSGHN VSQEAIAIKR MLEMGAVKNL TYF

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
61 NRS
131 NSS 129 - 140
LRNSSLGGVINK
Shared ( 2 )
310 NVS 303 - 319
SMAGSGHNVSQEAIAIK
Shared ( 5 )
303 - 320
SMAGSGHNVSQEAIAIKR
Shared ( 5 )
303 - 319
SMAGSGHNVSQEAIAIK
Shared ( 5 )
303 - 320
SMAGSGHNVSQEAIAIKR
Shared ( 5 )
329 NLT
Colon Kidney(Fut9 KO) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-)
Amide80 AAL(+) ConA RCA120 RCA120 RCA120
           
       
         
 
   
 
 
     
 
           
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05