GlycoProtDB ID - GPDB0007629


Protein Name Aspartic proteinase family member similar to renin
Protein Accession Number Q9JM79
Gene Napsa
Length 419
Status
release : 2016-12-05

Glycosylation Sites

Schema of N-glycosylation site(s) of the protein

Sequence

(__:Potential Sequon , N:Identified Site)

1
101
201
301
401
MSPLLLLLLC LLLGNLEPEE AKLIRVPLQR IHLGHRILNP LNGWEQLAEL SRTSTSGGNP SFVPLSKFMN TQYFGTIGLG TPPQNFTVVF DTGSSNLWVP
STRCHFFSLA CWFHHRFNPK ASSSFRPNGT KFAIQYGTGR LSGILSQDNL TIGGIHDAFV TFGEALWEPS LIFALAHFDG ILGLGFPTLA VGGVQPPLDS
MVEQGLLEKP VFSFYLNRDS EGSDGGELVL GGSDPAHYVP PLTFIPVTIP AYWQVHMESV KVGTGLSLCA QGCSAILDTG TSLITGPSEE IRALNKAIGG
YPFLNGQYFI QCSKTPTLPP VSSHLGGVWF NLTGQDYVIK ILQSDVGLCL LGFQALDIPK PAGPLWILGD VFLGPYVAVF DRGDKNVGPR VGLARAQSRS
TDRAERRTTQ AQFFKRRPG

N-glycosylation sites

Potential sites Identified Peptide*1
Position Sequon Position Sequence Unique or Shared
85 NFT
128 NGT 121 - 131
ASSSFRPNGTK
Shared ( 5 )
149 NLT
331 NLT
Kidney Kidney(Fut9 KO) Kidney(Fut9 WT) Liver Liver: b4GalT-I(+/+) Liver: b4GalT-I(-/-) Lung
AAL RCA120 AAL(+) AAL(+) AAL RCA120 SSA RCA120 RCA120 ConA RCA120
                     
                     
                     
*1:   _:Potential Sequon     :Asn (glycosylated)     :Gln (deaminated:pyroGlu)     :Met (oxidized)     :Cys (carbamidomethylated and deaminated)

External Links

Publications

Noro E, Togayachi A, Sato T, Tomioka A, Fujita M, Sukegawa M, Suzuki N, Kaji H, Narimatsu H Large-Scale Identification of N-Glycan Glycoproteins Carrying Lewis x and Site-Specific N-Glycan Alterations in Fut9 Knockout Mice. J Proteome Res 2015;14(9):3823-34 PMID:26244810
Sugahara D, Kaji H, Sugihara K, Asano M, Narimatsu H Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach Sci Rep 2012;2(): PMID:23002422
Kaji H, Shikanai T, Sasaki-Sawa A, et al Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB J Proteome Res 2012;11(9):4553-4566 PMID:22823882
Kaji H, Yamauchi Y, Takahashi N, Isobe T Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site--specific stable isotope tagging Nat Protocols 2007;1(6):3019-3027 PMID:17406563

Entry information

Entry version 2016-12-05
protein sequence version 2016-12-05
Entry status Latest version
Entry history

create : 2016-12-05